OCCUPATIONAL RISK OF HEPATITIS C AMONG DENTAL PROFESSIONAL BY ESTIMATION OF ANTIBODY TITRE FOR HEPATITIS C
- 1. Saveetha Dental College and Hospitals, Masilamani Nagar, Seneerkuppam Bypass Road, Poonamallee, Chennai, Tamilnadu
Description
Aim: To determine the levels of Hepatitis C antibody (anti-HCV) from blood samples among a cohort of dental professional using quantitative seroanalysis.
Objective: Determination of the antibody titre levels of HCV provides information of dental health professionals with adequate protection against the HCV antigen.
Background: Dental health care workers have an occupational risk hazard of acquiring blood-borne viral pathogens such as hepatitis C virus (HCV). The risk is maximised due to constant contact of blood from performing dental procedures on patients who may in turn be carrying the virus via needle-stick injuries and open wounds. HCV can be prevented by strict adherence to standard microbiological practices and techniques and the routine use of appropriate barrier precautions to prevent skin and mucous membrane exposure when handling blood and other body fluids of all patients in health care settings.
Materials and Method: The cohort study included 85 dental professionals (M=42, F=43) who were currently pursuing or had pursued their under graduation and/or post graduation in dentistry. Subjects were informed about the study and a written consent form was obtained. 2ml of blood was collected intravenously and transferred to a vaccutainer tube. The blood was then allowed to coagulate for half an hour and later centrifuged at 3000rpm for 5 mins. The supernatant liquid was pipetted, stored in Eppendorf tubes and incubated at 4˚C. The HCV MICROLISA® kit was used to detect the antibody titre in the serum sample as per the manufacturer's instructions. The processing of the samples was then performed using an automated ELISA analyser.
Result: The results of the study showed that all the subjects tested negative for Anti-HCV titres.
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References
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