Supplementary Figures and tables: Metabolic alteration of Trypanosoma cruzi during differentiation of epimastigote to trypomastigote forms.

Figure S1: Overlapping the total chromatographic profiles of organic acids extracted from parasites at different times (0, 24,48, and 72h) from cultivation on TAU3AAG medium, performed using the CR10 chromatographic program (Schimadzu, Kyoto, Japan). The extraction of organic acids was carried out as described in MM, and the separation was carried out on an Aminex XPX-87H column at a flow of 0.6 ml/min, using 0.07 mM H2SO4 as eluent. Detection was performed at 210 nm and a sensitivity of 0.08 AUFS. The arrows indicate the unidentified acids systematically located throughout the cultivation in the BHI medium (Fig. 4). Figure S2; Legend is similar to the previous one. Analysis of organic acids extracted from parasites at different times:. A (24h), B (72h), C (120h), D (168h), E (overlapping). Table S1 - Concentration of organic acids in different times of the differentiation of the Trypanosoma cruzi in TAU3AAG medium, obtained through the Aminex HPX 87H column. Table S2 - Concentration of the acids organic during the growth of the Trypanosoma cruzi in BHI medium, obtained through the Aminex HPX 87H column. Table S3- Correlation between the percentage of epimastigote and tripomastigote forms and the specific enzymatic activity of aldolase (Ald), hexokinase (Hk) and piruvate kinase (Pk) enzymes during the differentiation of the T. cruzi in TAU3AAG medium. Table S4- Specific enzymatic activity of the aldolase (Ald), hexokinase (Hk) and piruvate kinase (Pk) extracted from epimastigotes maintained in BHI medium