MiRNA-133a and MiRNA-25 3p and their relationship with some variables in serum of patients with Osteoporosis

Abstract

Osteoporosis is a chronic disease characterized by bone fragility that results in fractures and a variety of miRNAs are involved in osteoclast differentiation therefore, the current case control study aimed to estimate miRNA-133a and miRNA-25 3p in osteoporotic patients and evaluate relationship of these miRNAs with some variables including (calcium, vitamin D, BMD, smoking,  history of previous fracture and gender, this study conducted on fifty patients suffering from osteoporosis with age range between 50-88 years, other group  consist of 45 healthy individuals with an age range between 55-87 years included in this study as a control group. Blood samples used to extraction of miRNA-133a and miRNA-25 3p from the serum of patients and healthy control as a biomarker for osteoporosis were quantitated by using RT-PCR. Results:  miR-133a fold change was significantly upregulated   in serum of   osteoporotic patients and highest in patients group compared with control group, miR-133a highly significant difference in miR-133 among study groups (P < 0.001); while no significant difference in miR-25 among study groups (P = 0.295); although the level of patients groups was higher than that of control group. Receiver operator characteristic (ROC) curve  of  miR-133  was carried out and cutoff  value  was  >8.3  with  sensitivity, specificity, positive predictive value (PPV), negative predictive value (NPV)  and area under curve of 76%, 80%,82.6%, 72.7% and 0.815 (0.723- 906), while (ROC) curve analysis and  cutoff  value of miR-25 was >1.32  with  sensitivity, specificity, positive predictive value (PPV), negative predictive value (NPV) and Area under curve of 66%, 45%, 60%, 51.4% and 0.565 (0.444- 685).Conclusion: miRNA-133a is high sensitivity and Specificity in this study which was bushed to using them as a biomarker for osteoporosis diagnosis.