IRCCS Humanitas Research Hospital & Humanitas University
Published December 7, 2021 | Version v1
Dataset Restricted

Dataset related to article "Single-cell profiling identifies impaired adaptive NK cells expanded after HCMV reactivation in haploidentical HSCT"

Authors/Creators

  • 1. Unit of Clinical and Experimental Immunology, IRCCS Humanitas Research Hospital, Rozzano, Milan, Italy.
  • 2. Unit of Clinical and Experimental Immunology, IRCCS Humanitas Research Hospital, Rozzano, Milan, Italy AND BIOMETRA, Università degli Studi di Milano, Milan, Italy.
  • 3. Laboratory of Translational Immunology, IRCCS Humanitas Research Hospital, Rozzano, Milan, Italy.
  • 4. Institute of Genetic and Biomedical Research, UoS Milan, National Research Council, and Genomic Unit.
  • 5. Bone Marrow Transplant Unit, IRCCS Humanitas Research Hospital, Rozzano, Milan, Italy.
  • 6. Molecular Biology Section, Clinical Investigation Laboratory, IRCCS Humanitas Research Hospital, Milan, Italy.
  • 7. Bone Marrow Transplant Unit, IRCCS Humanitas Research Hospital, Rozzano, Milan, Italy AND Department of Biomedical Sciences, Humanitas University, Via Rita Levi Montalcini 4, 20072 Pieve Emanuele – Milan, Italy
  • 8. Institute for Virology, University Hospital Essen, University Duisburg-Essen, Essen, Germany.
  • 9. Department of Experimental Medicine, University of Genoa, Genoa, Italy.
  • 10. Laboratory of Translational Immunology AND Flow Cytometry Core, IRCCS Humanitas Research Hospital, Rozzano, Milan, Italy.

Description

This record contains data set related to the article "Single-cell profiling identifies impaired adaptive NK cells expanded after HCMV reactivation in haploidentical HSCT"

Haploidentical hematopoietic stem cell transplantation (h-HSCT) represents an efficient curative approach for patients affected by hematologic malignancies in which the reduced intensity conditioning induces a state of immunologic tolerance between donor and recipient. However, opportunistic viral infections greatly affect h-HSCT clinical outcomes. NK cells are the first lymphocytes that recover after transplant and provide a prompt defense against human cytomegalovirus (HCMV) infection/reactivation. By undertaking a longitudinal single-cell computational profiling of multiparametric flow cytometry, we show that HCMV accelerates NK cell immune reconstitution together with the expansion of CD158b1b2jpos/NKG2Aneg/NKG2Cpos/NKp30lo NK cells. The frequency of this subset correlates with HCMV viremia, further increases in recipients experiencing multiple episodes of viral reactivations, and persists for months after the infection. The transcriptional profile of FACS-sorted CD158b1b2jpos NK cells confirmed the ability of HCMV to deregulate NKG2C, NKG2A, and NKp30 gene expression, thus inducing the expansion of NK cells with adaptive traits. These NK cells are characterized by the downmodulation of several gene pathways associated with cell migration, the cell cycle, and effector-functions, as well as by a state of metabolic/cellular exhaustion. This profile reflects the functional impairments of adaptive NK cells to produce IFN-γ, a phenomenon also due to the viral-induced expression of lymphocyte-activation gene 3 (LAG-3) and programmed cell death protein 1 (PD-1) checkpoint inhibitors.

Notes

This data set is composed by .fcs flow cytometry files.

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Additional details

Related works

Is supplement to
Journal article: 10.1172/jci.insight.146973 (DOI)
Journal article: 34003794 (ean8)