Reliability of CNEURO hyssops for sample collection in the SARS-CoV-2 diagnosis
Creators
- 1. Immunology Department, Basic and Preclinical Science Institute "Victoria de Girón", Havana Medical Science University and System Biology Department, Center for Genetic Engineer and Biotechnology. Havana, Cuba.
- 2. System Biology Department, Center for Genetic Engineer and Biotechnology. Havana, Cuba.
- 3. Head of Science and Technology, General Hospital "Dr. Luis Díaz Soto". Havana, Cuba.
- 4. System Biology Department. Center for Genetic Engineering and Biotechnology. Havana, Cuba.
- 5. Head of Biomedical Research. Biomedical Research Direction. Center for Genetic Engineering and Biotechnology. Havana, Cuba.
Description
Abstract
Background: The detection of SARS-CoV-2 genetic material from nasopharyngeal swab samples by RT-PCR is the most specific and sensitive way to test suspected cases. However, factors such as the sampling process, type of hyssop used, and the anatomical area from which the sample is collected can distort the result and cause false negatives.
Objective: to evaluate the reliability of CNUERO hyssops for sample collection in the SARS-CoV-2 diagnosis versus IMPROSWAB hyssops.
Methods: To study the reliability of hyssops developed in Cuba for swabbing in the COVID-19 diagnosis, by comparing them to other hyssops successfully used for this task, 2 swabbing samples were obtained from each patient (136). One of these two samples was taken using the hyssops made in Cuba, while the other was taken employing another hyssop imported from Germany.
The positive detections between both hyssops were compared using the Fisher exact test. The result of the detection of each hyssop was evaluated and compared using the ROC curve.
Results: The use of CNEURO hyssops allowed detecting 45 out of 59 positive cases, while IMPROSAWAB hyssops detected 52 out of 59. Non-significant differences were detected between positive cases detected for each hyssop. The sensibility of sample detection using CNEURO hyssops was of 76.3 % and of 88.1 % when using IMPROSWAB hyssops. Hence, no significant differences are detected in the detection of cases using these two hyssops.
Conclusion: CNEURO hyssops are safe and reliable to be used for nasopharyngeal samples taking of COVID-19 patients.
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