Dataset for "Rapid saliva infrared spectroscopy analysis provides snapshot of physiological response to COVID-19: comprehensive in vitro, mouse and cohort studies with meta-analysis"
Creators
- 1. Precision & Systems Biomedicine Laboratory, QIMR Berghofer Medical Research Institute, Herston, Brisbane, QLD, Australia 4006
- 2. 2Biostatistics Unit, QIMR Berghofer Medical Research Institute, Herston, Brisbane, QLD, Australia 4006
- 3. Inflammation Biology Laboratory, QIMR Berghofer Medical Research Institute, Herston, Brisbane, QLD, Australia 4006
- 4. New South Wales Health Pathology-Royal Prince Alfred Hospital, Camperdown, NSW, Australia 2050
- 5. Agilent Technologies Australia, Mulgrave, VIC, Australia 3170
- 6. Lung Inflammation & Infection Laboratory, QIMR Berghofer Medical Research Institute, Herston, Brisbane, QLD, Australia 4006, and The Prince Charles Hospital, Brisbane, Australia
Description
ATR-FTIR spectra was acquired to detect and inspect physiological response to SARS-CoV-2 infection from saliva samples. Three experimental models were used to determine robustness of the technique.
In vitro experiment tested supernatant derived from SARS-CoV-2 infected, UV-inactivated SARS-CoV-2 infected, or media control Vero cells. Supernatant collected at 24hr and 48hr post-infection. Data related to Figure 1.
Mouse experiment tested oral lavage from mice exposed to SARS-CoV-2 or UV-inactivated SARS-CoV-2 virus. Lavage collected at 0, 2 and 4 days post-infection. Data related to Figure 2.
Human experiment tested saliva collected at three different sites (NSW, TPCH, QIMR). Patients were tested for SARS-CoV-2 by gold standard PCR methods to determine infection status. PCR results and FTIR spectra included. Data related to Figure 3 and 4.