Repair properties of the new combined cream dermalipoin on the model of extended scheduled wounds in rats

Kononenko N., Sheikhali A. Repair properties of the new combined cream dermalipoin on the model of extended scheduled wounds in rats. Journal of Education, Health and Sport. 2016;6(6):722-731. eISSN 2391-8306. DOI http://dx.doi.org/10.5281/zenodo.5655297

https://apcz.umk.pl/JEHS/article/view/36008  

The journal has had 7 points in Ministry of Science and Higher Education parametric evaluation. Part B item 755 (23.12.2015).

755 Journal of Education, Health and Sport eISSN 2391-8306 7

© The Author (s) 2016;

This article is published with open access at Licensee Open Journal Systems of Kazimierz Wielki University in Bydgoszcz, Poland

Open Access. This article is distributed under the terms of the Creative Commons Attribution Noncommercial License which permits any noncommercial use, distribution, and reproduction in any medium,

provided the original author(s) and source are credited. This is an open access article licensed under the terms of the Creative Commons Attribution Non Commercial License

(http://creativecommons.org/licenses/by-nc/4.0/) which permits unrestricted, non commercial use, distribution and reproduction in any medium, provided the work is properly cited.

This is an open access article licensed under the terms of the Creative Commons Attribution Non Commercial License (http://creativecommons.org/licenses/by-nc/4.0/) which permits unrestricted, non commercial

use, distribution and reproduction in any medium, provided the work is properly cited.

The authors declare that there is no conflict of interests regarding the publication of this paper.

Received: 02.06.2016. Revised 22.06.2016. Accepted: 29.06.2016.

REPAIR PROPERTIES OF THE NEW COMBINED CREAM DERMALIPOIN ON THE MODEL OF EXTENDED SCHEDULED WOUNDS IN RATS

N. Kononenko, A. Sheikhali

National University of Pharmacy, Kharkiv, Ukraine

Kononenko Nadiia - Doctor of medical sciences, professor, Department of Pathological Physiology, National University of Pharmacy, Kulikovskа 12, Kharkiv, 61002 Ukraine, Contact tel.: 066-251-74-04, E-mail: kononenkonn76@gmail.com

Sheikhali Ali Marseille - Post-graduate full-time education, Department of Pathological Physiology, National University of Pharmacy, Kulikovskа 12, Kharkiv, 61002 Ukraine, Contact tel.: 0734822707, E-mail: sheyhali@mail.ru

Abstract

The aim of the work was to study the reparative properties of the new combined cream on the model of stencil wounds in rats.

Materials and methods. Reparative properties of a new combined preparation in the form of a cream for the treatment of inflammatory and microbial skin diseases conditionally named Dermalipoin, which included: α-lipoic acid, urea, olive oil, tea tree oil, PEG-400, was investigated on the model of extruded stencil wounds in rats. The effectiveness of treatment was assessed using planimetric indicators, which included determining: the area of wounds, the period of epithelialization, the number of animals with wounds that healed at different times and the coefficient of the rate of wound healing in comparison with the "Rescuer Forte" balm. The severity of the inflammatory reaction was drawn from changes in peripheral blood parameters: blood clotting time, hemoglobin content, the number of erythrocytes and leukocytes, leukocyte formula. To assess the effect of the cream on reparative processes, the activity of enzymes-markers of cytolysis ALAT and ASAT in the blood serum of experimental animals was determined. To assess the intensity of destruction of tissue proteins in the blood serum of experimental animals, the urea content was determined.

Results. On the model of stencil wounds in rats, it was found that when applying the new cream Dermalipoin, rapid wound healing occurred: the coefficient of the rate of wound healing was 3,31 versus 2,79 and 1,45 in animals from the groups of the reference drug and control pathology, respectively (p≤0, 05).The study of the dynamics of biochemical parameters showed that the use of Dermalipoin cream was accompanied by a significant (compared to the control pathology) decrease in the activity of ALAT and ASAT, as well as a decrease in the content of urea in the blood serum of experimental animals on the ninth day of the experiment, which indicates a significant decrease in the severity of cytodestructive processes. Changes in hematological parameters indicate a decrease in the severity of the inflammatory process when using Dermalipoin cream and Rescuer Forte balm: at the end of the experiment, there was a significant decrease in the total number of leukocytes and an increase in hemoglobin levels.

Conclusions. It was found that on the model of squeezed out stencil wounds in rats, Dermalipoin cream has a pronounced wound-healing effect and, in terms of the severity of the effect, is not inferior to the comparison drug, the Rescuer Forte balm. Therefore, further study of the new cream as a potential wound-healing and anti-burn drug is promising.

Key words: wounds; α-lipoic acid; urea; olive oil; tea tree oil; repair.