Assessment the concentration of malondialdehyde MDA and polyubiquitin C Protein in patients with breast and gastric cancer

Abstract                                                          

Background: Cancer death rates are the second reason for death all over the world despite the expansion of cancer diagnosing and care the high number of cancer patients are increasing in developing countries. The oxidative stress and ubiquitin proteins plays important roles in tumor cells.

Aim of study: This study aimed to investigate the implications of oxidative stress in breast and gastric cancer patients compared to healthy control subjects, for this purpose; we measured MDA and polyubiquitin C protein in blood serum of the study groups.  

Methods: Twenty-two female of breast cancer and twenty samples of healthy female as control with ages (24-73) years; twenty patients of gastric cancer samples and twenty of healthy control with ages (32-73) years. The cancer patients groups were collected at Al-Amal National Hospital for Cancer Treatment in Baghdad/Iraq. A level of serum MDA was determined according to Buege and Aust method, while serum polyubiquitin C protein was determined by ELISA technique.

Results: The results exhibited significantly increase (p=0.005) and high significant increase (p= 0.0001) in mean value of serum MDA level for breast and gastric cancer patients, respectively as compared with healthy donors. Additionally, the results showed significantly elevated (p= 0.018) and (p= 0.008) in mean value of serum polyubiquitin C protein level for breast and gastric cancer patients, respectively as compared with healthy volunteers. Moreover, the ROC curve exhibited polyubiquitin C protein level a fair cancer predictor for breast cancer patients and a good cancer predictor for gastric cancer in the diagnosis of these diseases according to the area under the curve (AUC). MDA concentration and polyubiquitin C protein concentration in patients of breast and gastric cancer showed a high significant difference (p>0.01).

Conclusion:  The oxidative stress (serumMDA levels) in gastric cancer is higher than in breast cancer. The level of serum polyubiquitin C protein in breast and gastric cancer patients is higher than serum MDA levels. Polyubiquitin C protein can be used as a useful tumor biomarker for breast and gastric cancer especially for diagnosing gastric cancer.