Specificity of amide VI band (FTIR) in globular protein stability

Department of Physics, Kongu Engineering College, Perundurai-638 052, Tamilnadu, India

E-mail : veluphysics@yahoo.com

Department of Physics (DDE), Annamalai University, Annamalainagar-608 002, Tamilnadu, India

Department of Physics, Paavai College of Engineering, Namakkal-637 018, Tamilnadu, India

Generally stabilization of globular proteins are attained by adding cosolvent glucose to resist denaturation due to extremes of pH in both acidic and alkaline region. In this work, the Fourier Transform Infrared (FTIR) spectra were recorded for ovalbumin protein with and without cosolvent (glucose) at pH 2, 5, 7, 9 and 12 in phosphate buffer. The changes in the secondary structures (α-helix, β-sheets and others) of ovalbumin caused by pH and influence of glucose on them were studied by FTIR spectra. These spectral examinations are specifically made in the amide VI band region towards the secondary structure of proteins since it was not analyzed yet. This analysis verify the stability of protein have good agreement with studies reported previously.