PHARMACOLOGICAL INVESTIGATION STUDIES OF ETHANOLIC PLANT EXTRACTS OF ADIANTUM INCISUM AND SAGITTARIA TRIFOLIA IN THROMBOSIS AND INFLAMMATION USING K-CARRAGEENEAN ANIMAL MODEL

The present study was undertaken to investigate the anti-thrombotic and thrombolytic activity of Adiantum incisum forsk leaves extracts and Sagittaria trifolia linn root extracts.

Male Albino Wistar rats were isolated in six groups comprising six creatures in each group. In order to induce thrombosis and test the activity of both the plant extracts in vivo, the carrageenan induced tail thrombosis model was utilized. K-carrageenan dissolved in saline and the right hind paw of each rat was injected subplantar with 20mg/kg B.W of k-carrageenan 1 hour after each dose of both plants extracts200mg/kg of AI and 200mg/kg of ST respectively) separately and in combination (200mg/kg AI+200 mg/kg ST). The tail thrombus lengths were measured in centimeters with scale and photographed at 24, 48, and 72 hrs after carrageenan injections. After appearance of the thrombus in rat tail, the animals were administered with the respective treatment as per the assigned group for 29 days.

After the completion of study, the blood sample of animals was collected by retroorbital puncture and the serum was separated and send to laboratory for assessment of haematological parameters, the rats were sacrificed and their tails were extracted for histopathological examination.

In this study, the extracts of Adiantum incisum(200mg/kg) showed substantial decrease in the tail thrombus lengths and more significant response compared to Sagittaria trifolia extracts(200mg/kg) and combination of both extracts (200mg/kg of AI + 200mg/kg of ST). The results were obtained from both the extracts respectively and in combination but the extract of Adiantum incisum were more potent than others.

The results of the selected showed marked decrease in CRP levels with Adiantum incisum being most evident among all. CRP being a main biomarker for inflammation and thrombosis

Key Words: Thrombosis, k-carrageenan model, Adiantum incisum forsk, Sagittaria trifolia linn, Thrombolytic activity, Inflammation, CRP.