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Published October 10, 2021 | Version 1.1.0
Software Open

StrucGP: a software for structural interpretation of N-glycans on intact glycopeptides using tandem mass spectrometry data

  • 1. Northwest University

Description

We developed a software named StrucGP for de novo interpretation of N-glycan structures on intact glycopeptides using tandem mass spectrometry. StrucGP is capable of distinguishing different structure isoforms within the same glycan composition and identifying new glycan structures thanks to its database-independent strategy. In order to interpret glycopeptides precisely, FDR for peptide and glycan was estimated separately and 1% FDR cutoff was applied for both peptide and glycan parts.

Notes

--------------------------------------------------------------------------------------------- Updates v1.1.0 (10/09/2021) 1. A brand new software named GlycoVisualTool was released for visualizing and annotating glycopeptide spectra identified by StrucGP. 2. Besides Trysin, protease cleavage sites can be modified according to the experiment treatments. 3. Compared with v1.0, additional excel file termed MSFileName_AllSpetra.xlsx were added for saving analyzing parameters, filtered reason of spectral pairs and matched PSMs before screening FDR. --------------------------------------------------------------------------------------------- How to acquire StrucGP license to activate your StrucGP software: 1. Run main.exe file, If StrucGP works, you can get your MAC address in StrucGP GUI. 2. After getting your MAC address, you need to copy it next to "MAC" between the "####" chars. 3. Besides, in order to get closer contact with users. You need to fill all below items between the "####" chars. You can just send this README txt file to sun_glycolab@126.com after finish it and we will provide StrucGP license to you as soon as possible. 4.You will find a LIC file named StrucGP_license in our reply. Please put it in the same folder as the main.exe. The wrong path or license file may cause failure when you start program. 5. If there are any other errors when you activate or use StrucGP, Please contact with us via sun_glycolab@126.com/JiechenShenGlyco@163.com. ##################################################################### MAC: User Name: Your e-mail address: University or Organization name: Country or Region: Your Leader or Supervisor: Your leader's e-mail: #####################################################################

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GlycoVisualTool_v1.0.zip

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