Kinetochore life histories reveal an Aurora B dependent error correction mechanism in anaphase

Dataset of kinetochore tracks in human RPE1 cells showing chromosome dynamics and segregation from prometaphase through to anaphase as described in detail in Sen, Harrison, Burroughs and McAinsh, 2021, https://doi.org/10.1101/2021.03.30.436326 Tracks correspond to 3D time-lapse movies of Ndc80-eGFP and were acquired in the 488nm channel using 1\% laser power, 50 ms exposure time/z-plane, 93 z-planes, 307 nm z-step, which results in 4.7 s/z-stack time frame. Cells are subject to nocodazole arrest-and-release or equivalent treatment with DMSO as indicated in the folder names, and some cells are subject to additional treatment with ZM to inhibit Aurora B (also indicated in folder names). Tracks were produced using kinetochore tracking software, KiT v2.3 (see Armond et al., 2016, Bioinformatics), available from https://github.com/cmcb-warwick/KiT/