Published August 14, 2021 | Version v2
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Figure S1. Autophagy inhibitors in the autophagy reporter THP1-Difluo hLC3 cell line

Creators

  • 1. Faculty of Pharmacy, University of Ljubljana, SI-1000 Ljubljana, Slovenia
  • 2. Department of Haematology, University Medical Centre Ljubljana, SI-1000 Ljubljana, Slovenia

Description

Cells (5 ×105 cells/mL) were incubated with two concentrations of (a) mTOR activator MHY1485, (b) AMPK inhibitor dorsomorphin, (c) ULK1/2 inhibitor MRT68921, (d) PI3K class III inhibitor wortmannin,  (e) autophagosome-lysosome fusion inhibitor chloroquine, and (f) late-stage autophagy inhibitor bafilomycin A1 for 24 h. Autophagic flux was determined by measuring the fluorescence of RFP and GFP. The ratio of median fluorescence intensity of RFP to GFP (MFI) was normalized (nMFI) to the vehicle control (CTRL DMSO). Data are means ±SEM of 4 independent experiments, each carried out in duplicate.

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