Published April 2, 2021 | Version v.1
Journal article Open

Evaluation of Cyclin D1 expression by western blotting methods and immunohistochemistry in breast cancer patients

  • 1. Department for Radiation Oncology and Diagnostics, Institute of Oncology and Radiology of Serbia, 11 000 Belgrade, Pasterova 14, Serbia.
  • 2. Department of Medical Oncology, Institute of Oncology and Radiology of Serbia, Belgrade. 11 000 Pasterova 14, Serbia
  • 3. Department of Department of Pathophysiology, Univeristy of Kragujevac, Faculty of Medical Sciences, Svetozara Markovića 69, 34 000 Kragujevac, Serbia

Description

Purpose: Considering that cyclin D1 had a prognostic and clinical value for breast cancer patients, adequate measurement of cyclin D1 is necessary. Methods: In this investigation, we detect cyclin D1 expression in tumour and peritumoral tissue of breast cancer patients by Western blotting method and by immunohistochemistry. Results: Cyclin D1 expression decreased significantly with each advanced clinical stage of disease and tumour size. Also, patients without lymph node involvement, with positive hormone receptors and Luminal A type of tumours had significantly increased the expression of cyclin D1. We show that cyclin D1 expression correlates with longer RFS in the entire group of patients, in the group of ER-positive and in the group of HER2-negative patients. Patients who were both ER and cyclin D1 positive had a better prognosis. Conclusion: Taken together, our results, showing correlation of cyclin D1 with clinical stage, tumour size and lymph nodes, suggest that cyclin D1 expression, detected by Western blotting, could be considered as an additional marker for the staging of breast cancer, as well as a marker for longer RFS and survival in ER-positive breast cancer patients. 

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Evaluation of Cyclin D1 expression by western blotting methods and immunohistochemistry in breast cancer patients. J BUON 26-2-475.pdf

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Related works

Is identical to
34076995 (PMID)
Is part of
1107-0625 (ISSN)

Funding

Molecular, biochemical and immunological tumor diagnosis 175056
Ministry of Education, Science and Technological Development