Assessing the potential of nutraceuticals as geroprotectors on muscle performance and cognition in aging mice

Supplementary Figure 1. Assesment of latency and distance covered to find the target hole on the 6th day of training of Barnes-maze test following 4 weeks of krill oil supplementation . Traces of the time latency and distance trajectories of a control mouse (A-B) were significantly greater than those recorded for the krill oil fed counterpart (C-D). The time to find the target hole (A, C) was 59.0 sec vs 15.4 sec, whereas the distance covered (B,D) was 260.5 cm vs 127.1 cm for CTRL and Krill oil, respectively.

Supplementary Figure 2. The effects of nutraceutical administration on mitochondrial fission protein Drp1. (A) RT-qPCR analysis shows significant differences in the levels of Drp1 mRNA transcript expression in TA muscles of AX  but not in krill oil supplemented mice. Data were obtained from 4 mice/group. All results are shown as means ± SEM with * p < 0.02. (B) Representative Western blot against Drp1 (cat.no. 8570, Cell Signalling). The numbers on the gels indicate the number of animals studied.  (C) Quantification of expression levels of Drp1 show significantly decreased protein levels following nutraceutical supplementation when normalized to α-actinin used as an internal control. Data (+SEM) is from 2 independent experiments. ***p<0.001.

Video S1 and S2: The movement of a CTRL and a krill oil treated mouse on the 6^th day of Barnes-maze training protocol.