Phytochemical Studies of the Extracts of Mangifera indica Linn

In the recent times herbal medicine have gained high popularity both in developing and developed countries because of their natural origin and reduced side effects. More importantly, the claim that some plants have therapeutic action and are used for myriads of pathological conditions has generated more research interest globally. Mangifera indica known to possess varied medicinal properties in its back, leaves and fruits is a common example. In this study the phytochemical constituents in the ethylacetate and methanol leave extracts of M. indica were analyzed. The phytochemical screening results showed the presence of glycoside, saponin, flavonoids, tannins (condensed tannins), reducing sugar, alkaloids and terpenoids for methanol extract and glycoside, cardiac glycoside, saponins, flavonoids, tannins (hydrolysable tannins), terpenoids, reducing sugar, alkaloid and steroid for ethylacetate soluble fraction. Isolation was done with solvent extraction while purification was achieved with a chromatographic technique (HPLC). The structural elucidation of the pure compound isolated was attempted using mass spectroscopy and the presence of mangiferin in the methanol and ethylacetate soluble fraction was confirmed. However, the methanol fraction was discovered to have higher percentage of mangiferin considering the intensity of the peak in the HPLC-MS spectra.