ramp-pulse-zebrafish-heart
Creators
- 1. Idiap Research Institute
- 2. University of Bern
Description
Description
This dataset contains the raw data used in publication [1]. It features image series of the beating heart of a 48 hours post fertilization old Tg(actb2:LIFEACT-RFP) [2] transgenic zebrafish. Tg(actb2:LIFEACT-RFP) express red fluorescent proteins that bind to F-actin fibers. The images were acquired on an OpenSPIM microscope with an UMPLFLN 20XW semi-apochromat water dipping objective lens. The temporal illumination pattern alternated between ramp-illumination and pulse-illumination for each of the 100 frames of the series (ramp, pulse, ramp, pulse, etc., see [1]). The camera exposure time of each frame was 70ms, the pulse duration was 4ms (see [1] for details on the ramp characteristics).
References
[1] O. Mariani, F. Marelli, C. Jaques, A. Ernst, M. Liebling, "Unequivocal cardiac phase sorting from alternating ramp- and pulse- illuminated microscopy image sequences", IEEE 18th International Symposium on Biomedical Imaging (ISBI), 13-16 April 2021, in press
Files
MD5SUM.txt
Files
(38.1 MB)
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Additional details
Related works
- Cites
- Conference paper: 10.1109/ISBI48211.2021.9433858 (DOI)
Funding
- Platform for Reproducible Acquisition, Processing, and Sharing of Dynamic, Multi-Modal Data 206021_164022
- Swiss National Science Foundation
- The Cellular Basis of Cardiac Development Revealead by Live Imaging 310030E-164245
- Swiss National Science Foundation