Published February 20, 2014 | Version v1
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Data from: Genome- and transcriptome-assisted development of nuclear insertion/deletion markers for Calanus species (Copepoda: Calanoida) identification

  • 1. Faculty of Biosciences and Aquaculture; University of Nordland; 8049 Bodø Norway*
  • 2. Technical University of Denmark
  • 3. Grønlands Naturinstitut
  • 4. Plymouth Marine Laboratory
  • 5. University of Connecticut
  • 6. Aarhus University

Description

Copepods of the genus Calanus are key zooplankton species in temperate to arctic marine ecosystems. Despite their ecological importance, species identification remains challenging. Furthermore, the recent report of hybrids among Calanus species highlights the need for diagnostic nuclear markers in order to efficiently identify parental species and hybrids. Using Next Generation Sequencing analysis of both the genome and transcriptome from two sibling species, C. finmarchichus and C. glacialis, we developed a panel of 12 nuclear insertion/deletion markers. All the markers showed species specific amplicon length. Furthermore most of the markers were successfully amplified in other Calanus species allowing the molecular identification of C. helgolandicus, C hyperboreus and C. marshallae.

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Related works

Is cited by
10.1111/1755-0998.12241 (DOI)