Dryad
Published April 19, 2018 | Version v1
Dataset Open

Data from: Debugging diversity – a pan‐continental exploration of the potential of terrestrial blood‐feeding leeches as a vertebrate monitoring tool

Creators

  • 1. Curtin University
  • 2. University of Copenhagen
  • 3. Centre for Rainforest Studies at the School for Field Studies; Yungaburra Queensland Australia*
  • 4. Stony Brook University
  • 5. James Cook University
  • 6. Copenhagen Zoo
  • 7. University of East Anglia
  • 8. School for Field Studies
  • 9. University of Leeds
  • 10. American Museum of Natural History
  • 11. Leibniz Institute for Zoo and Wildlife Research
  • 12. University of Cambridge

Description

The use of environmental DNA (eDNA) has become an applicable non-invasive tool with which to obtain information about biodiversity. A sub-discipline of eDNA is iDNA (invertebrate-derived DNA), where genetic material ingested by invertebrates is used to characterise the biodiversity of the species that served as hosts. While promising, these techniques are still in their infancy, as they have only been explored on limited numbers of samples from only a single or a few different locations. In this study, we investigate the suitability of iDNA extracted from more than 3,000 haematophagous terrestrial leeches as a tool for detecting a wide range of terrestrial vertebrates across five different geographical regions on three different continents. These regions cover almost the full geographical range of haematophagous terrestrial leeches, thus representing all parts of the world where this method might apply. We identify host taxa through metabarcoding coupled with high-throughput sequencing on Illumina and IonTorrent sequencing platforms to decrease economic costs and workload and thereby make the approach attractive for practitioners in conservation management. We identified hosts in four different taxonomic vertebrate classes: mammals, birds, reptiles, and amphibians, belonging to at least 42 different taxonomic families. We find that vertebrate blood ingested by haematophagous terrestrial leeches throughout their distribution is a viable source of DNA with which to examine a wide range of vertebrates. Thus, this study provides encouraging support for the potential of haematophagous terrestrial leeches as a tool for detecting and monitoring terrestrial vertebrate biodiversity.

Notes

Australia_PSinfo_12s
Australia_PSinfo_16s
Australia_Tags
PeninsularMalaysia_PSinfo
AsiaMadagascar_Tags_16s
LaosVietnamDS1_PSinfo
LaosVietnamDS1_Pool1_merged.fastq
LaosVietnamDS1_Pool2_merged
LaosVietnamDS1_Pool3_merged
LaosVietnamDS1_Pool4_merged
LaosVietnamDS1_Pool5_merged
LaosVietnamDS1_Pool6_merged
LaosVietnamDS1_Pool7_merged
LaosVietnamDS1_Pool8_merged
LaosVietnamDS1_Pool9_merged
LaosVietnamDS1_Pool10_merged
LaosVietnamDS1_Pool11_merged
LaosVietnamDS1_Pool12_merged
LaosVietnamDS2_merged
LaosVietnamDS2_PSinfo
Madagascar_Pool1_merged
Madagascar_Pool2_merged
Madagascar_Pool3_merged
Madagascar_Pool4_merged
Madagascar_PSinfo
MalaysianBorneoDS1_Pool1_merged
MalaysianBorneoDS1_Pool2_merged
MalaysianBorneoDS1_PSinfo
MalaysianBorneoDS2_Pool1_merged
MalaysianBorneoDS2_Pool2_merged
MalaysianBorneoDS2_PSinfo
PeninsularMalaysia_merged
Australia_Pool1_merged
Australia_Pool2_merged
Australia_Pool3_merged

Files

AsiaMadagascar_Tags_16s.txt

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Additional details

Related works

Is cited by
10.1111/1755-0998.12912 (DOI)