Published June 10, 2021 | Version v1
Journal article Open

Changes in morphological and biochemical blood parameters in the postoperative period in experimental orthopedic surgeries in rabbits, treated with bone marrow aspirate, platelet-rich plasma and hydroxyapatite

  • 1. Assistant Professor Department of "Surgery, Radiology, Obstetrics, and Gynecology", Faculty of Veterinary Medicine, University of Forestry, Bulgaria
  • 2. Associated Professor and Head of "Department of Surgery, Radiology, Obstetrics, and Gynecology", Faculty of Veterinary Medicine, University of Forestry, Sofia, Bulgaria

Description

The evaluation of the morphological and biochemical blood parameters is extremely helpful after orthopedic procedures as it could be used as an indicator for the quality of the regeneration process. There were significant changes in the RBC count, amount of Hb and Hct in the postoperative period. We observed an increase in the total number of platelets as a result of the soft tissue and bone trauma, destruction of blood vessels, etc. All the changes in the post-surgical period regarding the biochemical parameters reflect the changes that occur in the macro-organism after anesthesia and surgery. Serum alkaline phosphatase differences in all groups is because of different activation of osteoblasts, producing specific bone alkaline phosphatase.

Files

MDS-V58-I1-C1-FEB-2021 (PP-1-6).pdf

Files (1.6 MB)

Name Size Download all
md5:19a1d67220a5084cf9f2d901379bc5c3
1.6 MB Preview Download

Additional details

References

  • Shafiei Z, Oryan A, Meimandi A, Bigham A. Histological, biomechanical and radiological evaluation of bone repair with human platelet rich plasma in rabbit model. - Zahedan journal of research in medical sciences,17(2):e1953; 2015.
  • Yin N., Wang Y., Ding L. et al. Platelet-rich plasma enhances the repair capacity of muscle-derived mesenchymal stem cells to large humeral bone defect in rabbits. - Scientific reports 10, 6771; 2020.
  • Ye L., Shu-Kui C., Long L., Ling Q., Xin-Luan W., Yu-Xiao L. Bone defect animal models for testing efficacy of bone substitute biomaterials. - Journal of orthopaedic translation, Vol. 3, Issue 3, 95-104; 2015.
  • Massányia M.,Kohút L., Argente M., Haloa M., Kováčik A., Kováčiková E., Ondruška L., Formicki G., Massány P. The effect of different sample collection methods on rabbit blood parameters. - Saudi journal of biological sciences, vol. 27, Issue 11, 3157-3160; 2020.
  • Toth C., Klarik Z., Kiss F., Toth E., Hargitai Z., Nemeth N. Early postoperative changes in hematological, erythrocyte aggregation and blood coagulation parameters after unilateral implantation of polytetrafluoroethylene vascular graft in the femoral artery of beagle dogs. - Acta Cir. Bras., vol. 29, issue 5; 2014.
  • Meurrens J., Steiner, T., Ponette, J., Janssen, H. A., Ramaekers, M., Wehrlin, J. P., Vandekerckhove, P., & Deldicque, L. Effect of Repeated Whole Blood Donations on Aerobic Capacity and Hemoglobin Mass in Moderately Trained Male Subjects: A Randomized Controlled Trial. - Sports medicine, 2(1), 43; 2016.
  • Otto J, Plumb J, Clissold E, Kumar S, Wakeham D, Schmidt W, Grocott M, Montgomery T. Hemoglobin concentration, total hemoglobin mass and plasma volume in patients: implications for anemia. - Haematologica, 102(9), 1477–1485; 2017.
  • Madaan G, Jairajpuri Z, Hajini F, Jetley S. Postoperative thrombocytosis: An unusual case report. - International journal of applied & basic medical research, 5(3), 225–227; 2015.
  • Lipman S, Marini R, Erdman S. A comparison of ketamine/xylazine and ketamine/xylazine/ acepromazine anesthesia in the rabbit. - Laboratory animal science. 40:395-398; 1990.
  • González A, Illera C, Silván G, Lorenzo P, Illera M. Changes in hepatic and renal enzyme concentrations and heart and respiratory rates in new zealand white rabbits after anesthetic treatments. - Contemporary topics in laboratory animal science, 41(6):30-2; 2002.
  • Nishizawa Y, Ohta H, Miura M. Guidelines for the use of bone metabolic markers in the diagnosis and treatment of osteoporosis. - Journal of bone and mineral metabolism, 31, 1–15; 2013.
  • Ajai S, Sabir A, Mahdi A, Srivastava N. Evaluation of serum alkaline phosphatase as a biomarker of healing process progression of simple diaphyseal fractures in adult patients. - International research journal of biological sciences, Vol. 2(2), 40-43; 2013.
  • Nazhta H, Omara R, Muna R, A Al Dahhana, Khaleefa R, Kareem S, Manual Z, Theia Z. Estimation of alkaline phosphatase enzymes level in the femoral transverse fract ures healing in rabbits. - Global proceedings repository american research foundation, pp. 97-107; 2019.