Vitamín D v úlohe reparácie DNA senescentných buniek poškodených peroxidom vodíka
Creators
- 1. Ústav Lekárskej chémie, biochémie a klinickej biochémie, LF UK, Bratislava
Description
SÚHRN
V rámci našich experimentov sme sa zamerali na sledovanie protektívnych účinkov vitamínu D na senescentné (starnúce) bunky MRC-5 v pasáži 21 (p21) a nesenescentné (mladé) bunky v pasáži 8 (p8) pred a po poškodení peroxidom vodíka. MTT testom sme zistili, že vitamín D zvýšil prežívanie buniek p8 nad 100 % v koncentrácii 20 µmol/L a 25 µmol/L. V prípade buniek p21 vitamín D zvýšil prežívanie buniek nad 100 % v koncentrácii 10 µmol/L a 25 µmol/L. Tieto zvýšenia však neboli signifikantné. Následne sme sledovali vplyv vitamínu D na poškodenie DNA buniek indukované peroxidom vodíka a zistili sme, že vitamín D vykazuje nielen protektívny, ale aj reparačný účinok. Tento účinok bol signifikantný v koncentráciách 1, 10, 20, 25 a 50 µmol/L.
ABSTRACT
In our experiments we focused on monitoring the protective effects of vitamin D on senescent MRC-5 cells at passage 21 (p21) and non-senescent cells at passage 8 (p8) before and after hydrogen peroxide damage. By MTT test we found that vitamin D increased the survival of p8 cells above 100 % at a concentration of 20 µmol/L and 25 µmol/L. In the case of p21 cells, vitamin D increased cell survival above 100 % at a concentration of 10 µmol/L and 25 µmol/L. However, these increases were not significant. Subsequently, we monitored the effect of vitamin D on hydrogen peroxide-induced DNA damage in cells and found that vitamin D has not only a protective but also a repair effect. This effect was significant at concentrations of 1, 10, 20, 25 and 50 µmol/L.
Notes
Files
Laboratórna Diagnostika XXVI_1_2021 48–52.pdf
Files
(393.9 kB)
Name | Size | Download all |
---|---|---|
md5:8aff75acb06ab842a7d06536ed82c434
|
393.9 kB | Preview Download |
Additional details
References
- Calder, P. C. and Kew, S. (2002): The im mune system: a target for functional foods? British Journal of Nutrition. doi: 10.1079/bjn2002682.
- Chin, Y. R. and Toker, A. (2010): The Actin-Bundling Protein Palladin Is an Akt1-Specific Substrate that Regulates Breast Cancer Cell Migration. Molecular Cell. doi: 10.1016/j. molcel.2010.02.031.
- Collins, A. R. et al. (1996): Oxidative damage to DNA: Do we have a reliable biomarker? Environmental Health Perspectives. doi: 10.1289/ehp.96104s3465.
- Fraga, C. G. et al. (1990): Oxidative damage to DNA during aging: 8-Hydroxy-2'-deoxyguanosine in rat organ DNA and urine. Proceedings of the National Academy of Sciences of the United States of America. doi: 10.1073/pnas.87.12.4533.
- Liu, Q. et al. (2014): Role of AKT signaling in DNA repair and clinical response to cancer therapy. Neuro-Oncology. doi: 10.1093/neuonc/nou058.
- Meehan, M. and Penckofer, S. (2014): The Role of Vitamin D in the Aging Adult. Journal of Aging and Gerontology. doi: 10.12974/2309-6128.2014.02.02.1.
- Morales, J. C. et al. (2014): Review of poly (ADP-ribose) polymerase (PARP) mechanisms of action and rationale for targeting in cancer and other diseases. Critical Reviews in Eukaryotic Gene Expression. doi: 10.1615/CritRevEukaryotGene Expr.2013006875.
- Nair-Shalliker, V., Armstrong, B. K., Fenech, M. (2012): Does vitamin D protect against DNA damage ? Mutation Research—Fundamental and Molecular Mechanisms of Mutagenesis. doi: 10.1016/j.mrfmmm.2012.02.005.
- Newberry, S. J. et al. (2014): Vitamin D and Calcium: A Systematic Review of Health Outcomes (Update). Evidence report/ technology assessment. doi: 10.23970/AHRQEPCERTA217.
- Paes, J. E. and Ringel, M. D. (2008): Dysregulation of the Phosphatidylinositol 3-Kinase Pathway in Thyroid Neoplasia. Endocrinology and Metabolism Clinics of North America. doi: 10.1016/j.ecl.2008.01.001.
- Pfeffer, P. E. et al. (2018): Effects of Vitamin D on inflammatory and oxidative stress responses of human bronchial epithelial cells exposed to particulate matter. PLoS ONE. doi: 10.1371/journal.pone.0200040.
- Songyang, Y. et al. (2021): Effect of vitamin D on malignant behavior of non-small cell lung cancer cells. Gene. doi: 10. 1016/j.gene.2020.145309.
- Xie, Y. et al. (2019): PI3K/Akt signaling transduction pathway, erythropoiesis and glycolysis in hypoxia (Review). Molecular Medicine Reports. doi: 10.3892/mmr.2018.9713.