Preclinical Study of Nanostructured Cubic Liquid Crystalline Formulating of Ulva Fascista Bioactive Polysaccharides against Hepatocirrhosis

From our previous study, the aqueous soluble polysaccharides isolated from cold aqueous extract of Ulva fasciata was prepared and represented as the most of its bioactive constituents. The polysaccharides were formulated as cubosomal nanoparticles for use as anti-hepatocirrhosis drugs. The formulations were characterized by their encapsulation efficiency, particle size, zeta potential and in vitro release. The selected formulation was subjected to a preclinical study. Serum biomarkers enzymes (aspartate and alanine aminotransferases and alkaline phosphatase) were proved the efficacy of the polysaccharides loaded cubosome similar to the reference drug; silymarin in addition to its safety on liver. The histopathological examination was conducted to document the biochemical results.