makefile and scripts for: Time-resolved structured illumination microscopy reveals key principles of Xist RNA spreading

Xist RNA directs the process of X-chromosome inactivation in mammals by spreading in cis along the chromosome from which it is transcribed and recruiting chromatin modifiers to silence gene transcription. To elucidate mechanisms of Xist RNA cis-confinement, we established a sequential dual-color labeling, super-resolution imaging approach to trace individual Xist RNA molecules over time, RNA-SPLIT (Sequential Pulse Localization Imaging over Time), enabling us to define fundamental parameters of spreading. This publication contains the different scripts required for image processing and analysis of RNA-SPLIT datasets.