Published April 27, 2021 | Version 1.0.0
Software Open

nf-core/clipseq: nf-core/clipseq 1.0.0 - Ianthine Pelican

  • 1. The Francis Crick Institute
  • 2. Science for Life Laboratory
  • 3. Francis Crick Institute

Description

Initial release of nf-core/clipseq, created with the nf-core template.

Pipeline summary
  1. Adapter and quality trimming (Cutadapt)
  2. Pre-mapping to e.g. rRNA and tRNA sequences (Bowtie 2)
  3. Genome mapping (STAR)
  4. UMI-based deduplication (UMI-tools)
  5. Crosslink identification (BEDTools)
  6. Bedgraph coverage track generation (BEDTools)
  7. Peak calling (multiple options):
    • iCount
    • Paraclu
    • PureCLIP
    • Piranha
  8. Motif detection (DREME)
  9. Quality control:
    • Sequencing quality control (FastQC)
    • Library complexity (Preseq)
    • Regional distribution (RSeQC)
  10. Overall pipeline run and QC summaries and peak calling comparisons (MultiQC)

Files

nf-core/clipseq-1.0.0.zip

Files (2.3 MB)

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Additional details

Related works