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Published January 25, 2021 | Version v1
Journal article Open

SUMCASTEC_210125_Microsecond Pulsed Electric Fields: An Effective Way to Selectively Target and Radiosensitize Medulloblastoma Cancer Stem Cells

Creators

  • 1. Division of Health Protection Technologies, Italian National Agency for Energy New Technologies and Sustainable Economic Development
  • 2. Department of Ecological and Biological Sciences, University of Tuscia,
  • 3. Energy Efficiency Department, ENEA

Description

Purpose

Cancer stem cells constitute an endless reserve for the maintenance and progression of tumors, and they could be the reason for conventional therapy failure. New therapeutic strategies are necessary to specifically target them. In this context, microsecond pulsed electric fields have been selected to expose D283Med cells, a human medulloblastoma cell line resulted to be rich in cancer stem cells, and normal human astrocytes.

Methods

We analyzed in vitro different endpoints at different times after microsecond pulsed electric field exposure, such as permeabilization, reactive oxygen species generation, cell viability/proliferation, cell cycle, and clonogenicity, as well as the expression of different genes involved in cell cycle, apoptosis, and senescence. Furthermore, the response of D283Med cells exposed to microsecond pulsed electric fields was validated in vivo in a heterotopic mouse xenograft model.

Files

Microsecond Pulsed Electric Fields An Effective Way to Selectively Target and Radiosensitize Medulloblastoma Cancer Stem Ce.pdf

Files (1.6 MB)

Additional details

Funding

European Commission
SUMCASTEC – Semiconductor-based Ultrawideband Micromanipulation of CAncer STEm Cells 737164