Published August 28, 2014 | Version v1.0
Dataset Open

Molecular architecture of the 40S⋅eIF1⋅eIF3 translation initiation complex

Authors/Creators

  • 1. Department of Biology, Institute of Molecular Biology and Biophysics, ETH Zurich, Otto-Stern-Weg 5, 8093 Zurich, Switzerland
  • 2. Department of Biology, Institute of Molecular Systems Biology, ETH Zurich, Auguste-Piccard-Hof 1, 8093 Zurich, Switzerland
  • 3. Department of Bioengineering and Therapeutic Sciences, Department of Pharmaceutical Chemistry, and California Institute for Quantitative Biosciences (QB3), University of California, San Francisco, UCSF MC 2552, Byers Hall Room 503B, 1700 4th Street, San Francisco, CA 94158-2330, USA
  • 4. Department of Biology, Institute of Molecular Systems Biology, ETH Zurich, Auguste-Piccard-Hof 1, 8093 Zurich, Switzerland; Faculty of Science, University of Zurich, 8006 Zurich, Switzerland

Description

Eukaryotic translation initiation requires the recruitment of the large, multiprotein eIF3 complex to the 40S ribosomal subunit. Using X-ray structures of all major components of the minimal, six-subunit Saccharomyces cerevisiae eIF3 core, together with cross-linking coupled to mass spectrometry, we were able to use IMP to position and orient all eIF3 components on the 40S•eIF1 complex, revealing an extended, modular arrangement of eIF3 subunits.

For more information about how to reproduce this modeling, see https://salilab.org/40S-eIF1-eIF3 or the README file.

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Additional details

Related works

Is supplement to
https://github.com/integrativemodeling/40s-eif1-eif3/tree/v1.0 (URL)
10.1016/j.cell.2014.07.044 (DOI)
https://salilab.org/40S-eIF1-eIF3 (URL)