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Published February 26, 2021 | Version v1
Journal article Open

Metadata standards and practical guidelines for specimen and DNA curation when building barcode reference libraries for aquatic life

Authors/Creators

  • 1. INRAE, Thonon, France
  • 2. King Abdullah University of Science and Technology, Thuwal, Saudi Arabia
  • 3. AZTI, Pasaia, Spain|King Abdulaziz University, Jeddah, Saudi Arabia
  • 4. INRAE, Thonon les Bains, France
  • 5. Slovak Academy of Sciences, Bratislava, Slovakia
  • 6. University of Minho, Braga, Portugal
  • 7. Swiss Centre for Applied Ecotoxicology, Lausanne, Switzerland
  • 8. University of Szczecin, Szczecin, Poland
  • 9. Institut Pasteur, Paris, France
  • 10. University of Geneva, Geneva, Switzerland
  • 11. Freie Universität Berlin, Berlin, Germany
  • 12. Swedish University of Agricultural Sciences, Uppsala, Sweden
  • 13. University of Duisburg-Essen, Essen, Germany
  • 14. Meise Botanic Garden, Meise, Belgium
  • 15. Royal Botanic Garden Edinburgh, Edinburgh, United Kingdom|Institute for Food and Agricultural Research and Technology, Sant Carles de la Rápita, Spain
  • 16. INRAE, Rennes, France
  • 17. University of Nantes, Nantes, France
  • 18. University of Geneva, Geneve, Switzerland|ID-Gene ecodiagnostics, Geneva, Switzerland|Institute of Oceanology, Polish Academy of Sciences, Sopot, Poland
  • 19. Sorbonne Université, Paris, France
  • 20. University of Angers, Angers, France
  • 21. Institute for Food and Agricultural Research and Technology, Sant Carles de la Rápita, Spain
  • 22. SCIMABIO Interface, Thonon-les-Bains, France
  • 23. Musée national d'histoire naturelle de Luxembourg, Luxembourg, Luxembourg
  • 24. Norwegian University of Science and Technology, Trondheim, Norway

Description

DNA barcoding and metabarcoding is increasingly used to effectively and precisely assess and monitor biodiversity in aquatic ecosystems. As these methods rely on data availability and quality of barcode reference libraries, it is important to develop and follow best practices to ensure optimal quality and traceability of the metadata associated with the reference barcodes used for identification. Sufficient metadata, as well as vouchers, corresponding to each reference barcode must be available to ensure reliable barcode library curation and, thereby, provide trustworthy baselines for downstream molecular species identification. This document (1) specifies the data and metadata required to ensure the relevance, the accessibility and traceability of DNA barcodes and (2) specifies the recommendations for DNA harvesting and for the storage of both voucher specimens/samples and barcode data.

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