STABILITY INDICATING RP-UPLC METHOD FOR SIMULTANEOUS ESTIMATION OF GLECAPREVIR AND PIBRENTASVIR IN BULK AND TABLET DOSAGE FORMS

A novel, simple, accurate and rapid method has been developed and validated for and in bulk and in tablet dosage form. In RP-UPLC method, elution was achieved in isocratic mode using combination of Glecaprevir and Pibrentasvir in the ratio of 0.1% OPA: Acetonitrile (60:40) using a HH C18 (100×2.1mm, 1.8µm) column. The flow rate was 1ml/min and detection was done at 260nm.The method was validated for linearity, accuracy, precision, specificity and sensitivity as per ICH norms. The developed and validated method was successfully used for the quantitative analysis of commercially available dosage form.

Keywords: Glecaprevir, Pibrentasvir, RP-UPLC, Validation.