The study of the prooxidant-antioxidant equilibrium in the kidney tissue and blood on the background of ceruloplasmin administration under the conditions of acute kidney injury
Description
Zamorskii I., Unhuryan T., Batranovska S. The study of the prooxidant-antioxidant equilibrium in the kidney tissue and blood on the background of ceruloplasmin administration under the conditions of acute kidney injury. Journal of Education, Health and Sport. 2020;10(11):298-305. eISSN 2391-8306. DOI http://dx.doi.org/10.12775/JEHS.2020.10.11.030
https://apcz.umk.pl/czasopisma/index.php/JEHS/article/view/JEHS.2020.10.11.030
https://zenodo.org/record/4545781
The journal has had 5 points in Ministry of Science and Higher Education parametric evaluation. § 8. 2) and § 12. 1. 2) 22.02.2019.
© The Authors 2020;
This article is published with open access at Licensee Open Journal Systems of Nicolaus Copernicus University in Torun, Poland
Open Access. This article is distributed under the terms of the Creative Commons Attribution Noncommercial License which permits any noncommercial use, distribution, and reproduction in any medium,
provided the original author (s) and source are credited. This is an open access article licensed under the terms of the Creative Commons Attribution Non commercial license Share alike.
(http://creativecommons.org/licenses/by-nc-sa/4.0/) which permits unrestricted, non commercial use, distribution and reproduction in any medium, provided the work is properly cited.
The authors declare that there is no conflict of interests regarding the publication of this paper.
Received: 16.10.2020. Revised: 30.10.2020. Accepted: 30.11.2020.
UDC: 615.36:615.275:616.61-002
THE STUDY OF THE PROOXIDANT-ANTIOXIDANT EQUILIBRIUM IN THE KIDNEY TISSUE AND BLOOD ON THE BACKGROUND OF CERULOPLASMIN ADMINISTRATION UNDER THE CONDITIONS OF ACUTE KIDNEY INJURY
I. Zamorskii, T. Unhuryan, S. Batranovska
Bukovinian State Medical University
Abstract
Acute kidney injury (AKI) remains a relevant medical problem. The promising direction of the AKI treatment is the use of the antioxidants that inhibit free radical processes and prevent the destruction of cell membranes within pathological processes. Ceruloplasmin (CP) is the main antioxidant of blood plasma, that demonstrates membrane protecting effects and antitoxic activity.
The aim of the study was to establish the influence of a single-dose administration of ceruloplasmin on the processes of the peroxidation in the kidney tissue and blood under the conditions of acute kidney injury.
Materials and methods. The influence of ceruloplasmin on the prooxidant-antioxidant equilibrium in the kidney tissue and blood was studied in an experiment on white non-line rats under the conditions of rhabdomyolysis-induced acute kidney injury. The acute kidney injury was modeled by intramuscular administration of 50% glycerol solution at a dose of 8 mg/kg. Ceruloplasmin was administered intraperitoneally at a dose of 7 mg/kg/day. The level of main indicators of prooxidant-antioxidant balance in blood and kidney tissue was determined in 24 h after glycerol administration for induction of acute kidney injury.
Research results and their discussion. There was a deep disruption of the prooxidant-antioxidant equilibrium as a result of the acute kidney injury. AKI causes an activation of the prooxidant processes by increasing the level of the malonic dialdehyde and the oxidized proteins in the kidney tissue and blood. AKI also reduces the antioxidant protection by decreasing the activity of the antioxidant enzymes in the kidneys and blood plasma.
A single-dose administration of ceruloplasmin improved antioxidant protection and reduced peroxidation of lipids and proteins. There was a decrease in levels of malondialdehyde and oxidative modification of proteins. At the same time, the antioxidant protection intensified due to an increase in the activity of glutathione peroxidase and catalase in the kidneys and blood plasma.
Conclusions. A single administration of ceruloplasmin influences prooxidant-antioxidant balance, reducing the content of lipid and protein peroxidation products and increasing the activity of antioxidant enzymes.
Key words: acute kidney injury; oxidative stress; prooxidant-antioxidant balance; antioxidants; ceruloplasmin.
Files
33211.pdf
Files
(371.1 kB)
Name | Size | Download all |
---|---|---|
md5:e631ddad0ecaf36e27693622b26dc498
|
371.1 kB | Preview Download |