Quantitative one- and two-dimensional (13)C spectra of microcrystalline proteins with enhanced intensity.

We recorded quantitative, uniformly enhanced one- and two-dimensional 13C spectra of labelled microcrystalline proteins. The approach takes advantage of efficient equilibration of magnetization by low-power proton irradiation using Phase Alternated Recoupling Irradiation Schemes and benefits simultaneously from uniform sensitivity enhancement due to efficient spin exchange that can overcome T1(13C) constraints and the presence of heteronuclear Overhauser effects. Graphical Abstract