IRCCS Humanitas Research Hospital & Humanitas University
Published October 12, 2020 | Version v1
Dataset Restricted

Dataset related to article "Two subsets of stem-like CD8+ memory T cell progenitors with distinct fate commitments in humans".

Authors/Creators

  • 1. Laboratory of Translational Immunology, Humanitas Clinical and Research Center – IRCCS, Rozzano, Milan, Italy
  • 2. Innovative Immunotherapies Unit, Division of Immunology, Transplantation, and Infectious Diseases, IRCCS San Raffaele Scientific Institute, Milan, Italy
  • 3. Vaccine and Infectious Disease Division, Fred Hutchinson Cancer Research Center, Seattle, WA, USA
  • 4. Central European Institute of Technology, Brno, Czech Republic
  • 5. Humanitas Flow Cytometry Core, Humanitas Clinical and Research Center – IRCCS, Rozzano, Milan, Italy
  • 6. Institute of Genetic and Biomedical Research, UoS Milan, National Research Council, Rozzano, Milan, Italy AND Genomic Unit, Humanitas Clinical and Research Center – IRCCS, Rozzano, Milan, Italy
  • 7. Department of Biomedical Sciences, Humanitas University, Pieve Emanuele, Milan, Italy
  • 8. Unit of Clinical and Experimental Immunology, Humanitas Clinical and Research Center – IRCCS, Rozzano, Milan, Italy AND Department of Medical Biotechnologies and Translational Medicine, University of Milan, Milan, Italy
  • 9. Center for Cancer Research, National Cancer Institute, Bethesda, MD, USA AND Regensburg Center for Interventional Immunology, Regensburg, Germany AND University of Regensburg, Regensburg, Germany
  • 10. St. Jude Children's Research Hospital, Memphis, TN, USA
  • 11. Division of Cancer and Genetics, Cardiff University School of Medicine, Cardiff, UK
  • 12. Division of Infection and Immunity, Cardiff University School of Medicine, Cardiff, UK
  • 13. Division of Infection and Immunity, Cardiff University School of Medicine, Cardiff, UK AND Systems Immunity Research Institute, Cardiff University School of Medicine, Cardiff, UK
  • 14. Shemyakin and Ovchinnikov Institute of Bioorganic Chemistry, Moscow, Russia AND Pirogov Russian National Research Medical University, Moscow, Russia AND Center of Life Sciences, Skolkovo Institute of Science and Technology, Moscow, Russia
  • 15. Laboratory of Translational Immunology, Humanitas Clinical and Research Center – IRCCS, Rozzano, Milan, Italy AND Humanitas Flow Cytometry Core, Humanitas Clinical and Research Center – IRCCS, Rozzano, Milan, Italy

Description

This record contains data related to article “Two subsets of stem-like CD8+ memory T cell progenitors with distinct fate commitments in humans”.

ABSTRACT

T cell memory relies on the generation of antigen-specific progenitors with stem-like properties. However, the identity of these progenitors has remained unclear, precluding a full understanding of the differentiation trajectories that underpin the hetero- geneity of antigen-experienced T cells. We used a systematic approach guided by single-cell RNA-sequencing data to map the organizational structure of the human CD8+ memory T cell pool under physiological conditions. We identified two previously unrecognized subsets of clonally, epigenetically, functionally, phenotypically and transcriptionally distinct stem-like CD8+ memory T cells. Progenitors lacking the inhibitory receptors programmed death-1 (PD-1) and T cell immunoreceptor with Ig and ITIM domains (TIGIT) were committed to a functional lineage, whereas progenitors expressing PD-1 and TIGIT were committed to a dysfunctional, exhausted-like lineage. Collectively, these data reveal the existence of parallel differentiation programs in the human CD8+ memory Tcell pool, with potentially broad implications for the development of immunotherapies and vaccines.

Notes

FUNDING This work was funded by the European Research Council (ERC-2014-STG PERSYST no. 640511 to E.L.) and by the Associazione Italiana per la Ricerca sul Cancro (AIRC IG 20676 to E.L.). Additional support was provided by the Associazione Italiana per la Ricerca sul Cancro (AIRC IG 21567 to D.M.), the Italian Ministry of Health (Bando Ricerca Finalizzata PE-2016-02363915 to D.M.), the Intramural Research Fund of the Humanitas Clinical and Research Center (5 ×1000 2019 Program to D.M.) and Cancer Research UK (C17199/A18246/A29202 to D.M.B.). G.G., G.D.S., S.P. and E.S. were supported by Fellowships from the Fondazione Italiana per la Ricerca sul Cancro-Associazione Italiana per la Ricerca sul Cancro (FIRC-AIRC). A.N.D. and M.M. were supported by the Ministry of Education, Youth, and Sports of the Czech Republic (CEITEC 2020 LQ1601). D.A.P. was supported by a Wellcome Trust Senior Investigator Award (100326/Z/12/Z). D.M.C. was supported by the Ministry of Health of the Russian Federation (0908300057056). The purchase of a FACSSymphony A5 was defrayed in part by a grant from the Italian Ministry of Health (Agreement 82/2015).

Files

Restricted

The record is publicly accessible, but files are restricted to users with access.

Request access

If you would like to request access to these files, please fill out the form below.

You need to satisfy these conditions in order for this request to be accepted:

This set of data is accessible only upon request because it includes sensitive data.

Please write your request to biblioteca@humanitas.it.

You are currently not logged in. Do you have an account? Log in here

Additional details

Related works

Is supplement to
10.1038/s41590-020-0791-5 (DOI)
33046887 (PMID)

Funding

European Commission
PERSYST - Generation and maintenance of long-lived memory T cells in humans 640511
Wellcome Trust
The immunopathogenesis of Epstein-Barr virus-associated malignancies. 100326