Integrated immunoassay-based broad detection of multi-class mycotoxin
Authors/Creators
- 1. Institute of Apicultural Research, Chinese Academy of Agricultural Sciences, Key Laboratory of Bee Products for Quality and Safety Control, Ministry of Agriculture, Beijing, People's Republic of China
- 2. College of Life Science, Yantai University, Yantai, Shandong, People's Republic of China
- 3. College of Life Sciences, Linyi University, Linyi, Shandong, People's Republic of China
- 4. Department of Veterinary Pharmacology and Toxicology, College of Veterinary Medicine, China Agricultural University, Beijing, People's Republic of China
Description
Europium (Eu3+) and horse radish peroxidase (HRP) are highly promising biosensors due to its excellent physical and chemical properties. The two biosensors are widely applied in time-resolved fluoroimmunoassay (TRFIA) and chemiluminescence enzyme-linked immunosorbent assay, respectively. In this work, the two technologies were integrated with parallel input and parallel output (PI–PO) characters. Eu3+ was adopted as probe I in TRFIA for deoxynivalenol and de-epoxyldexoylnivalenol with a limit of detection (LOD) of 4.74 and 4.56 ng/mL, while HRP was performed as probe II in CL-ELISA for FB1 with an LOD of 0.088 ng/mL. Compared to the traditional individual assay, this method exhibited fast and broad detection characteristics by integrating two immunoassays into one.
Files
Integrated immunoassay based broad detection of multi class mycotoxins.pdf
Files
(1.7 MB)
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