Journal article Open Access

Pod indehiscence in common bean is associated with the fine regulation of PvMYB26

Valerio Di Vittori; Elena Bitocchi; Monica Rodriguez; Saleh Alseekh; Elisa Bellucci; Laura Nanni; Tania Gioia; Stefania Marzario; Giuseppina Logozzo; Marzia Rossato; Concetta De Quattro; Maria L Murgia; Juan José Ferreira; Ana Campa; Chunming Xu; Fabio Fiorani; Arun Sampathkumar; Anja Fröhlich; Giovanna Attene; Massimo Delledonne; Björn Usadel; Alisdair R Fernie; Domenico Rau; Roberto Papa

In legumes, pod shattering occurs when mature pods dehisce along the sutures, and detachment of the valves promotes seed dispersal. In Phaseolus vulgaris (L)., the major locus qPD5.1-Pv for pod indehiscence was identified recently. We developed a BC4/F4 introgression line population and narrowed the major locus down to a 22.5 kb region. Here, gene expression and a parallel histological analysis of dehiscent and indehiscent pods identified an AtMYB26 orthologue as the best candidate for loss of pod shattering, on a genomic region ~11 kb downstream of the highest associated peak. Based on mapping and expression data, we propose early and fine up-regulation of PvMYB26 in dehiscent pods. Detailed histological analysis establishes that pod indehiscence is associated with the lack of a functional abscission layer in the ventral sheath, and that the key anatomical modifications associated with pod shattering in common bean occur early during pod development. We finally propose that loss of pod shattering in legumes resulted from histological convergent evolution and that it is the result of selection at orthologous loci.

The study was part of the PhD research work of the first author (VD) and he is grateful to the Doctoral School of the Polytechnic University of Marche (UNIVPM) and to the Forschungszentrum Jülich (IBG-2) that co-funded the PhD scholarship. This work was supported by the BRESOV project, funded from the European Union's Horizon 2020 research and innovation program under grant agreement no. 774244, the Ministero dell'Istruzione, dell'Università e della Ricerca (MIUR, Italian Government; grant number RBFR13IDFM_001, FIRB Project 2013), and the Università Politecnica delle Marche. ARF and SA acknowledge the European Union project PlantaSyst (SGA-CSA no. 664621 and no. 739582 under FPA no. 664620).
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