An Integrative Model of Carbon and Nitrogen Metabolism in a Common Deep-Sea Sponge (Geodia barretti)

ACKNOWLEDGEMENTS Titus Rombouts and Pieter Slot (UvA) and Sharyn Ossebaar (NIOZ) are acknowledged for their analytical assistance with the nutrient measurements of the incubation experiments. We thank Asimenia Gavriilidou and Detmer Sipkema (WUR, SponGES) for their input on the microbial genome in G. barretti. We thank last Hans Tore Rapp (UiB) for the excellent project coordination. We would like to thank CF and CR for their valuable and constructive reviews. AUTHOR CONTRIBUTIONS AK developed the model, performed the data analyses and model analyses, prepared the figures, and wrote the manuscript. MB and JG designed and conducted the incubation experiments, performed nutrient analyses, and contributed to the writing of the manuscript. SV contributed to model conceptualization and development. JM acquired funding and contributed to the conceptualization of the model, interpretation, and writing of the manuscript. MM contributed to the conceptualization of the model, interpretation, and writing. SL and SM contributed data for model development. KS contributed technically with model implementation and analyses. DO designed the model, contributed to model development and interpretation, and writing of the manuscript. All authors contributed to the article and approved the submitted version. SUPPLEMENTARY MATERIAL The Supplementary Material for this article can be found online at: https://www.frontiersin.org/articles/10.3389/fmars.2020.596251/full#supplementary-material CONFLICT OF INTEREST The authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest. DATA AVAILABILITY STATEMENT The dataset of Leys et al. (2018) is available at University of Alberta Education and Research Archive: doi: 10.7939/R3057D48V. The dataset of Bart et al. (2020b) will be made available at Pangaea. The model input files and R scripts are available on Zenodo: doi: 10.5281/zenodo.4139792. FUNDING This research has been performed in the scope of the EU SponGES project, which received funding from the European Union's Horizon 2020 Research and Innovation Program under grant agreement no. 679849. Further support included ERC starting grant agreement no. 715513 to JG and the Netherlands Earth System Science Center to JM. This document reflects only the authors' views, and the Executive Agency for Small and Medium-sized Enterprises (EASME) is not responsible for any use that may be made of the information it contains.