Technical note Open Access

Cryosectioning of fixed and frozen tissue

Lepine, Paula; Tabatabaei, Mahdieh; Da Silva, Ronan; Durcan, Thomas M.

This protocol will provide a standardized procedure on methods developed for cryosectioning of fixed or fresh frozen tissue in Optimal Cutting Temperature (OCT) using a motorized cryostat. This SOP also outlines minimum assessment criteria that should be in place to evaluate the quality and integrity of frozen tissue sections and troubleshooting steps when issues with sectioning arise.

Files (1.0 MB)
Name Size
EDDU-017-01_Cryosectioning of frozen tissue_v1.0_merged.pdf
md5:4f5e5d6e3147766e9d0624b27a7ddac9 		1.0 MB Download
447
326
views
downloads
All versions This version
Views 447447
Downloads 326326
Data volume 328.3 MB328.3 MB
Unique views 356356
Unique downloads 291291
More info on how stats are collected.
Indexed in
Publication date:
January 20, 2021
DOI:
10.5281/zenodo.4451592

Zenodo DOI Badge

DOI 

10.5281/zenodo.4451592

Markdown 

[![DOI](https://zenodo.org/badge/DOI/10.5281/zenodo.4451592.svg)](https://doi.org/10.5281/zenodo.4451592)

reStructedText 

.. image:: https://zenodo.org/badge/DOI/10.5281/zenodo.4451592.svg
   :target: https://doi.org/10.5281/zenodo.4451592

HTML 

<a href="https://doi.org/10.5281/zenodo.4451592"><img src="https://zenodo.org/badge/DOI/10.5281/zenodo.4451592.svg" alt="DOI"></a>

Image URL 

https://zenodo.org/badge/DOI/10.5281/zenodo.4451592.svg

Target URL 

https://doi.org/10.5281/zenodo.4451592

Keyword(s):
cryosectioning tissue OCT embedding Cryostat
Communities:
License (for files):
Creative Commons Attribution 4.0 International

Versions

Version V1.0 10.5281/zenodo.4451592 Jan 20, 2021
Cite all versions? You can cite all versions by using the DOI 10.5281/zenodo.4451591. This DOI represents all versions, and will always resolve to the latest one. Read more.

Share

Cite as

Export