Addressable Nanoantennas with Cleared Hotspots for Single-Molecule Detection on a Portable Smartphone Microscope
Creators
- 1. Department of Chemistry and Center for NanoScience, Ludwig-Maximilians-Universität München, Butenandtstr. 5-13, 81377 München, Germany
- 2. Institute for Physical and Theoretical Chemistry - NanoBioScience and Braunschweig Integrated Centre of Systems Biology (BRICS), Technische Universität Braunschweig, Braunschweig, Germany
- 3. Department of Chemical and Biomolecular Engineering, North Carolina State University, Raleigh, NC, 27695, United States
- 4. Electrical & Computer Engineering Department, Bioengineering Department, California NanoSystems Institute (CNSI), and Department of Surgery, University of California, Los Angeles, California 90095, United States
- 5. Institut für Halbleitertechnik, Laboratory for Emerging Nanometrology LENA, TU Braunschweig, Langer Kamp 6a/b, 38106 Braunschweig, Germany
- 6. Département de Physique - Photonic Nanosystems, Université de Fribourg - Faculté des Sciences et Médicine, Chemin de Musée 3, CH-1700 Fribourg, Switzerland
Description
The advent of highly sensitive photodetectors and the development of photostabilization strategies made detecting the fluorescence of single molecules a routine task in many labs around the world. However, to this day, this process requires cost-intensive optical instruments due to the truly nanoscopic signal of a single emitter. Simplifying single-molecule detection would enable many exciting applications, e.g. in point-of-care diagnostic settings, where costly equipment would be prohibitive. Here, we introduce addressable NanoAntennas with Cleared HOtSpots (NACHOS) that are scaffolded by DNA origami nanostructures and can be specifically tailored for the incorporation of bioassays. Single emitters placed in the NACHOS emit up to 461-fold (average of 89±7-fold) brighter enabling their detection with a customary smartphone camera and an 8-US-dollar objective lens. To prove the applicability of our system, we built a portable, battery-powered smartphone microscope and successfully carried out an exemplary single-molecule detection assay for DNA specific to antibiotic-resistant Klebsiella pneumonia „on the road “. Here we demonstrate the raw data on which our findings based on.
Files
caDNAno_NACHOS.json
Files
(2.0 GB)
| Name | Size | Download all |
|---|---|---|
|
md5:6f8997a2afe2671070ab2cd71c6e51b5
|
590.9 kB | Preview Download |
|
md5:445a555502626401d40a7e67ea120893
|
1.8 GB | Download |
|
md5:4db8e1608d47ed4db78d49d2f29feb1c
|
205.2 MB | Download |
|
md5:acbb5c4cfda966b7de7263daeb22f0a7
|
984 Bytes | Download |
|
md5:8488986ae1e25c1f95d6749ea340b02a
|
6.4 MB | Download |