Supplementary data to accompany Information flow, cell types and stereotypy in a full olfactory connectome

Supplemental file 1

Layers assigned by the probabilistic graph traversal model. bodyId refers to neurons’ unique ID in ne- uPrint. layer mean contains the mean layer after 10,000 iterations of the main model (Figure 2). layer - olf mean and layer th mean contain the mean layers from running the traversal model with ORNs and THN/HRNs, respectively (Figure S2).

S1 hemibrain neuron layers.csv

Supplemental file 2

Sensory meta-information related to each glomerulus. Columns: glomerulus (canonical name for one of the 51 olfactory + 7 thermo/hygrosensory antennal lobe glomeruli), laterality (whether the glomerulus receives bilateral or only unilateral innervation from ALRNs), expected cit (a citation that describes the expected number of RNs in this glomerulus), expected RN female 1h (number of expected RNs in one hemi- sphere), expected RN female SD (standard deviation in the expected number of RNs), missing (qualitative assessment of glomeruli truncation), RN frag (if the RNs in that glomerulus are fragmented), receptor (the OR or IR expressed by cognate ALRNs (Bates et al., 2020; Task et al., 2020)), odour scenes (the general ‘odour scene(s)’ which this glomerulus may help signal (Mansourian and Stensmyr, 2015; Bates et al., 2020)), key ligand(the ligand that excites the cognate ALLRN or receptor the most, based on pooled data from multiple studies (Mu ̈nch and Galizia, 2016)), valence (the presumed valence of this odour chan- nel (Badel et al., 2016)). Exists as hemibrain glomeruli summary in our R package hemibrainr.

S2 hemibrain olfactory information.csv

Supplemental file 3

File listing all identified antennal lobe receptor neurons (ALRNs) in the hemibrain, including information shown in neuPrint. See above for column explanations. Exists as rn.info in our R package hemibrainr.

S3 hemibrain ALRN meta.csv

Supplemental file 4

All the hemibrain neurons we have classed as antennal lobe local neurons (ALLNs). See above for column explanations. Exists as alln.info in our R package hemibrainr.

S4 hemibrain ALLN meta.csv

Supplemental file 5

All the hemibrain neurons we have classed as antennal lobe projection neurons (ALPNs). See above for column explanations. In addition, across dataset cluster refers to the clustering with left and right FAFB PNs; is canonical indicates whether that ALPN is one of the well studied “canonical” uPNs. Exists as pn.info in our R package hemibrainr.

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S5 hemibrain ALPN meta.csv

Supplemental file 6

All the hemibrain neurons we have classed as third-order olfactory neurons (TOONs) including lateral horn neurons (LHNs), as well as wedge projection neurons (WEDPNs), lateral horn centrifugal neurons (LHCENT) and other projection neuron classes (Figure 1). See above for column explanations. Exists as ton.info in our R package hemibrainr.

S6 hemibrain TOON meta.csv

Supplemental file 7

All the hemibrain neurons we have classed as neurons that descend to the ventral nervous system (DNs). See above for column explanations. Exists as dn.info in our R package hemibrainr.

S8 hemibrain DN meta.csv

Supplemental file 8

The root point in hemibrain voxel space, for each hemibrain neuron. This is either the location of the soma, or the tip of a severed cell body fibre tract, where possible. Exists as hemibrain somas in our R package hemibrainr.

S8 hemibrain root points.csv

Supplemental file 9

The start points for different neuron compartments. Nodes downstream of this position in the 3D structure of the neuron indicated with bodyid, belong to the compartment type designated by Label. A product of running flow centrality on hemibrain neurons, exists as hemibrain splitpoints in our R package hemi- brainr.

S9 hemibrain compartment startpoints.csv

Supplemental file 10

3D triangle mesh for the hemibrain surface as a .obj file. This mesh was generated by first merging individual ROI meshes from neuPrint and then filling the gaps in between in a semi-manual process. It also exists as hemibrain.surf in our R package hemibrainr.

S10 hemibrain raw.obj

Supplemental file 11

3D meshes of 51 olfactory + 7 thermo/hygrosensory antennal lobe glomeruli for the hemibrain volume, generated from ALRN presynapses.

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Note that hemibrain coordinate system has the anterior-posterior axis aligned with the Y axis (rather than the Z axis, which is more commonly observed).

S11 hemibrain AL glomeruli meshes RN-based.zip

Supplemental file 12

3D meshes of 51 olfactory + 7 thermo/hygrosensory antennal lobe glomeruli for the hemibrain volume, generated from ALPN presynapses.

Note that hemibrain coordinate system has the anterior-posterior axis aligned with the Y axis (rather than the Z axis, which is more commonly observed).

These meshes are also available as hemibrain al.surf in our R package hemibrainr. S12 hemibrain AL glomeruli meshes PN-based.zip