Neuronal cell labelled with anti PSD95 and homer antibodies - sample image for software testing of Easy SODA

Hippocampal neurons in culture seeded on glass coverslip. This 16 bits confocal picture of hippocampal neuron in culture has been taken at 63x (1024x1024 pixels - pixel size 92,45 nm) in sequential mode with two channels : one dedicated to the PSD95 protein, and the other one to Homer protein. Those protein are expressed at glutamatergic post-synaptic sites and can be found close within Post Synaptic Density. This is a good sample to test colocalization and association analysis. The homer channel can be used both to infere neuronal shape thanks to hk-means segmentation (http://icy.bioimageanalysis.org/protocol/easy-cell-shape-with-hk-means/), or to detect clusters thanks to wavelet segmentation (http://icy.bioimageanalysis.org/protocol/rainbow-spot-detector-scale-finder/). This sample image can be used with Easy SODA protocol (Standard Object Distance Analysis) on Icy software (http://icy.bioimageanalysis.org/protocol/easy-soda-2-colors-1-image/) to detect association between PSD95 and homer clusters.