Comparative nephrotoxic effect on rats of different pathogens

Levitsky A. P., Stepan V. T., Pustovoit P. I. Comparative nephrotoxic effect on rats of different pathogens. Journal of Education, Health and Sport. 2020;10(10):212-218. eISSN 2391-8306. DOI http://dx.doi.org/10.12775/JEHS.2020.10.10.019

https://apcz.umk.pl/czasopisma/index.php/JEHS/article/view/JEHS.2020.10.10.019

https://zenodo.org/record/4309864

The journal has had 5 points in Ministry of Science and Higher Education parametric evaluation. § 8. 2) and § 12. 1. 2) 22.02.2019.

© The Authors 2020;

This article is published with open access at Licensee Open Journal Systems of Nicolaus Copernicus University in Torun, Poland

Open Access. This article is distributed under the terms of the Creative Commons Attribution Noncommercial License which permits any noncommercial use, distribution, and reproduction in any medium,

provided the original author (s) and source are credited. This is an open access article licensed under the terms of the Creative Commons Attribution Non commercial license Share alike.

(http://creativecommons.org/licenses/by-nc-sa/4.0/) which permits unrestricted, non commercial use, distribution and reproduction in any medium, provided the work is properly cited.

The authors declare that there is no conflict of interests regarding the publication of this paper.

Received: 12.10.2020. Revised: 16.10.2020. Accepted: 30.10.2020.

UDK 616.61:616.34:616.35:612.3:577.15

COMPARATIVE NEPHROTOXIC EFFECT ON RATS OF DIFFERENT PATHOGENS

A. P. Levitsky1, V. T. Stepan2, P. I. Pustovoit3

1Odessa National Academy of Food Technologies

2Bukovina State Medical University, Chernovtsy

3Municipal Institution «Odessa Regional Clinical Hospital»

Abstract

Aim. Compare the nephrotoxicity of different pathogens.

Methods. Nephrotoxicity was assessed in rats treated with the following pathogens: hydrazine sulfate, prednisolone, cyclophosphamide, lincomycin and lipopolysaccharide, according to the degree of increase in biochemical markers of inflammation: elastase activity and malondialdehyde (MDA) content.

Result. A significant increase in the level of both inflammatory markers in the kidneys was established, the most pronounced under the influence of hydrazine sulfate. The recalculation of the percentage increase in the level of markers per 1 mg of the pathogen (specific pro-inflammatory activity, SPIA) showed the highest SPIA in lipopolysaccharide, especially after oral applications, exceeding SPIA for hydrazine sulfate by a factor of thousands.

Conclusion. The pro-inflammatory effect on kidneys of many non-microbial toxicants can be realized through the formation of lipopolysaccharide by lysis of gram-negative bacteria.

Key words: kidneys; toxins; lipopolysaccharide; markers of inflammation.