Rapid, simple and validated reverse phase method for analysis of glimepiride from capsules

Glimepiride, a blood sugar controlling molecule have been analyzed from a marketed capsule consisting of glimepiride and pioglitazone using a reversed phase isocratic method. The method was developed on 150 mm × 4.6 mm, 5 µ C18 column. The mobile phase consisted of sodium dihydrogen o-phosphate pH 6.0: acetonitrile (60:40 v/v). The compounds gave a good UV response at 220 nm, hence the detection wavelength. The method was found to be highly specific for glimepiride in presence of Pioglitazone and other constituents of the capsule. The developed method is simple, precise, robust and stands validated as per ICH guidelines.