Rectal Firmicutes, Actinobacteria and Gammaproteobacteria phyla fitness belong to post mortem intervals in the terrestrial environment death victims evaluation in Malang, East Java, Indonesia

The increasing of urbanization will lead the increasing violence-related death, like traffic accident, murdered and suicide which contribute to 20 leading causes of death. Post mortem interval (PMI) estimation is important information for suspicious death. Microbes have important role for decomposition of bodies. By evaluate the certain bacteria will know the post mortem interval because the community of bacteria have certain condition regarding to the composition. The aim of this study is to figure out the community of Firmicutes, Actinobacteria and Gammaproteobacteria from rectal swab post mortem interval of the terrestrial environment in Malang, East Java, Indonesia. We used Wistar carcass as a model for human decomposition. Identification of bacterial phyla using Real Time PCR (qPCR) method after cultured in nutrient broth solution. The results showed that Firmicutes has dominant composition in fresh stage (0-24 hours) and in advance decomposition at 120 hours after death. Actinobacteria was dominant in the early decomposition, observed from the 48 to 72 hours after death. While Gammaproteobacteria was dominant in the end of fresh stage and early decomposition stage when bloating time occur.