Published May 16, 2020 | Version v1
Journal article Open

Optical activity of lysozyme in solution at 532 nm via signal-reversing cavity ring-down polarimetry

Creators

  • 1. IESL-FORTH
  • 2. University of Crete
  • 3. University of Ioannina

Description

An improved optical cavity-based polarimetry method is employed to measure the optical activity of lysozyme in water solution, in the concentration range of 0–2 mg/ml. We employ a signal reversing technique, which gives the absolute optical rotation, without needing to remove the sample for a null measurement. We report an absolute sensitivity limit on the order of 0.1 mdeg, corresponding to a detection limit of <50 μg/ml for a sample volume lower than 50 μL, thus surpassing the sensitivity of existing commercial polarimeters. We discuss how these sensitivity levels can be further improved using existing methods and technologies.

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Additional details

Funding

European Commission
ULTRACHIRAL – Ultrasensitive chiral detection by signal-reversing cavity polarimetry: applications to in-situ proteomics, single-molecule chirality, HPLC analysis, medical diagnostics, and atmospheric studies 737071