Metabolic signatures of Cryptosporidium parvum-infected HCT-8 cells and impact of selected metabolic inhibitors on C. parvum infection under physioxia and hyperoxia
Creators
- 1. Institute of Parasitology, Biomedical Research Center Seltersberg, Justus Liebig University Giessen
- 2. ulrich.gaertner@anatomie.med.uni-giessen.de
- 3. klaus.Failing@vetmed.uni-giessen.de
- 4. daugschies@vetmed.uni-leipzig.de
- 5. sybille.Mazurek@vetmed.uni-giessen.de
- 6. carlos.r.hermosilla@vetmed.uni-giessen.de
- 7. anja.taubert@vetmed.uni-giessen.de
Description
Most of in vitro researches on cryptosporidiosis have been performed under non-physiological oxygen conditions. Such situation has generated a lack of knowledge about C. parvum inference on the metabolism of its host cell under physiological oxygen concentrations, becoming a missing piece of puzzle to understand how C. parvum really modulates the metabolic profile of its host cell in order to replicate. To fill this gap we here comparatively performed metabolic analyses on C. parvum inference on the metabolism of its host cells under physiological oxygen conditions and under hyperoxic conditions, the last one representing the standard situation to the date.
In addition, considering the found metabolic conversion rates in the supernatants of C. parvum-infected cells, we selectively targeted the most critically parasite-dependent upregulated pathways, confirming glucolysis as pivotal for parasite replication. Likewise, we here report a C. parvum dependence on glutaminolysis and lactate extrusion via MCT for infection development.
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