Preprint Open Access

MicroRNA-148b-3p and MicroRNA-25-3p are overexpressed in fetuses with late-onset fetal growth restriction

Morales-Roselló, José; García Gimenez, José Luis; Martinez Priego, Llucia; González-Rodríguez, Daymé; Mena-Mollá, Salvador; Maquieira Catalá, Angel; Loscalzo, Gabriela; Buongiorno, Silvia; Jakaite, Vaidile; Cañada Martínez, Antonio José; Perales Marín, Alfredo

Objective: to describe a miRNA profile characteristic of late-onset growth restriction (FGR) and to investigate those pathways involved in their biochemical action.

Material and methods: In a prospective study, 25 fetuses: 16 normal and 9 with FGR (estimated fetal weight <10th centile plus cerebroplacental ratio <0,6765 MoM) were evaluated with Doppler ultrasound after 36 weeks. Afterwards, for every fetus, plasma from umbilical vein blood was collected at birth, miRNA was extracted, and full miRNA sequencing was performed. Subsequently, comparisons were done in order to obtain those miRNA that were differentially expressed.

Result: FGR fetuses expressed upregulation of two miRNA: miR-25-3p and especially miR-148b-3p, a miRNA directly involved in Schwann cell migration, neuronal plasticity and energy metabolism (p=0.0072, p=0.0013).

Conclusions: FGR fetuses express a different miRNA profile, which includes overexpression of miR-miR-25-3p and miR-148b-3p. This information might improve our understanding of the pathophysiological processes involved in late-onset FGR. Future validation and feasibility studies will be required to propose miRNAs as a valid tool in  the diagnosis and management of FGR.

This is the pre-peer reviewed version of the following article: Morales-Roselló J, García-Giménez J, L, Martinez Priego L, González-Rodríguez D, Mena-Mollá S, Maquieira Catalá A, Loscalzo G, Buongiorno S, Jakaite V, Cañada Martínez A, J, Perales Marín A: MicroRNA-148b-3p and MicroRNA-25-3p Are Overexpressed in Fetuses with Late-Onset Fetal Growth Restriction. Fetal Diagn Ther 2020;47:665-674 (DOI: 10.1159/000507619). The copyright holder is the publisher. The final, published version is available at Funding sources: 1-iPlacenta project, European Union's Horizon 2020 Research and Innovation Programme, MarieSkłodowska-Curie grant agreement Number 765274, 2- VLC-Biomed Grant, Universidad de Valencia & Universidad Politécnica de Valencia, 3- Clinical Study Protocol X213220 Xoma, Hospital Universitario y Politécnico La Fe, 4- Charity Run "9 km 9 meses", Instituto de Investigación Sanitaria La Fe and Ayuntamiento de Torrente, Valencia, Spain.
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