Published September 17, 2020 | Version 1
Dataset Open

Combining StarDist and TrackMate example 2 - T cell dataset

  • 1. Department of Pathology and Laboratory Medicine, Children's Hospital of Philadelphia Research Institute, Philadelphia, PA 19104, USA
  • 2. Faculty of Science and Engineering, Cell Biology, Åbo Akademi University, 20520 Turku, Finland

Description

Description: Contains a StarDist example training dataset, a test dataset, and the StarDist model generated using ZeroCostDL4Mic (see https://github.com/HenriquesLab/ZeroCostDL4Mic/wiki/Stardist)

Training dataset: 209 Paired microscopy images (brightfield) and corresponding masks

Microscopy data type: brightfield microscopy and masks obtained via manual segmentation (see https://github.com/HenriquesLab/ZeroCostDL4Mic/wiki/Stardist for details about the segmentation)

Microscope: Imaging was done using a 10x phase contrast objective at 37°C on a Zeiss Axiovert 200M microscope equipped with an automated X-Y stage and a Roper EMCCD camera. Time-lapse images were collected every 30 sec for 10 min using SlideBook 6 software (Intelligent Imaging Innovations).

File format: .tif (16-bit for brightfield images and 8 and 16-bit for the masks)

Image size: 1024x1024 (Pixel size: 645 nm)

Files

T cell dataset.zip

Files (331.4 MB)

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Additional details

Related works

Is documented by
3290-7931 (ISSN)