Video/Audio Open Access

AFM Surface Coating

Sumbul, Fidan; Claire Valotteau; Felix Rico

AFM Surface Coating - AFMBioMed Summer School 2020

This 7 minutes video tutorial shows how to properly label, handle, incubate (coat), rinse and store AFM surfaces (cantilevers and coverslips) during a multi-stage coating process.

Files (414.9 MB)
Name Size
414.9 MB Download
  • Sumbul F., Marchesi A., Takahashi H., Scheuring S., Rico F. (2018) High-Speed Force Spectroscopy for Single Protein Unfolding. In: Lyubchenko Y. (eds) Nanoscale Imaging. Methods in Molecular Biology, vol 1814. Humana Press, New York, NY

  • Jobst, M. A., Schoeler, C., Malinowska, K., & Nash, M. A. (2013). Investigating receptor-ligand systems of the cellulosome with AFM-based single-molecule force spectroscopy. JoVE (Journal of Visualized Experiments), (82), e50950.

  • Zimmermann, J. L., Nicolaus, T., Neuert, G., & Blank, K. (2010). Thiol-based, site-specific and covalent immobilization of biomolecules for single-molecule experiments. nature protocols, 5(6), 975-985.

  • Otten, M., Ott, W., Jobst, M. A., Milles, L. F., Verdorfer, T., Pippig, D. A., ... & Gaub, H. E. (2014). From genes to protein mechanics on a chip. Nature methods, 11(11), 1127-1130.

  • Becke, T. D., Ness, S., Sudhop, S., Gaub, H. E., Hilleringmann, M., Schilling, A. F., & Clausen-Schaumann, H. (2018). Covalent immobilization of proteins for the single molecule force spectroscopy. JoVE (Journal of Visualized Experiments), (138), e58167.

  • Yang, B., Liu, Z., Liu, H., & Nash, M. A. (2020). Next Generation Methods for Single-Molecule Force Spectroscopy on Polyproteins and Receptor-Ligand Complexes. Frontiers in Molecular Biosciences, 7.



All versions This version
Views 180180
Downloads 5858
Data volume 24.1 GB24.1 GB
Unique views 160160
Unique downloads 5656


Cite as