Published August 28, 2020 | Version v1
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Protocol for ultra performance liquid chromatography-mass spectrometry N-glycan analysis of the native and desialylated human apo-transferrin

Creators

  • 1. Faculty of Pharmacy and Biochemistry, University of Zagreb, Ante Kovačića 1, 10000 Zagreb, Croatia

Description

Experimental protocol for for ultra performance liquid chromatography-mass spectrometry N-glycan analysis of the native and desialylated human apo-transferrin.

Supplemental information for the published paper:

Friganović, Tomislav; Tomašić, Antonela; Šeba, Tino; Biruš, Ivan; Kerep, Robert; Borko, Valentina; Šakić, Davor; Gabričević, Mario; Weitner, Tin: Low-pressure chromatographic separation and UV/Vis spectrophotometric characterization of the native and desialylated human apo-transferrin // Heliyon, 7 (2021), 9; e08030, 8 doi:10.1016/j.heliyon.2021.e08030

 

Notes

This work was supported by funding from the Croatian Science Foundation grant UIP-2017- 05-9537 - Glycosylation as a factor in the iron transport mechanism of human serum transferrin (GlyMech), as well as the European Structural and Investments funding for the "Croatian National Centre of Research Excellence in Personalized Healthcare" (contract #KK.01.1.1.01.0010) and "Centre of Competences in Molecular Diagnostics" (contract #KK.01.2.2.03.0006).

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Transferrin - protocol for UPLC-MS N-glycan analysis.pdf

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