Assembly and comparison of two closely related Brassica napus genomes
Creators
- 1. School of Plant Biology, The University of Western Australia, Stirling Highway, Crawley, WA 6009, Australia
- 2. School of Plant Biology, The University of Western Australia, Stirling Highway, Crawley, WA 6009, Australia, Australian Centre for Plant Functional Genomics, School of Agriculture and Food Sciences, University of Queensland, St. Lucia, QLD 4072, Australia
- 3. Australian Centre for Plant Functional Genomics, School of Agriculture and Food Sciences, University of Queensland, St. Lucia, QLD 4072, Australia
- 4. chool of Plant Biology, The University of Western Australia, Stirling Highway, Crawley, WA 6009, Australia
- 5. National Key Laboratory of Crop Genetic Improvement, Key Laboratory of Rapeseed Genetic Improvement, Ministry of Agriculture P. R. China, Huazhong Agricultural University, Wuhan, China
- 6. Department of Biology, University of York, York, UK
- 7. Organization and Evolution of Plant Genomes, Unité de Recherche en Génomique Végétale, Unité Mixte de Recherche 1165 (Institut National de Recherche Agronomique, Centre National de la Recherche Scientifique, Université Evry Val d'Essonne), Evry 91057, France
- 8. National Key Laboratory of Crop Genetic Improvement, Key Laboratory of Rapeseed Genetic Improvement, Ministry of Agriculture P. R. China, Huazhong Agricultural University, Wuhan, China, Southern Cross Plant Science, Southern Cross University, Lismore, NSW 2480, Australia
Description
Here we present the de novo assembly of the B. napus cultivar Tapidor and comparison with an improved assembly of the B. napus cultivar Darmor-bzh. Both cultivars were annotated using the same method to allow comparison of gene content. We identified genes unique to each cultivar and differentiate these from artefacts due to variation in the assembly and annotation. We demonstrate that using a common annotation pipeline can result in different gene predictions, even for closely related cultivars, and repeat regions which collapse during assembly impact whole genome comparison. After accounting for differences in assembly and annotation, we demonstrate that the genome of Darmor-bzh contains a greater number of genes than the genome of Tapidor.
Notes
Files
Darmor_v81_Tapidor_Ningyou_SNPs.zip
Files
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