A new adenylyl cyclase, putative disease resistance RPP13-like protein 3, participates in abscisic acid-mediated heat stress resistance in maize

This dataset include figures and supplementary material for the manuscript entitled "A new adenylyl cyclase, putative disease resistance RPP13-like protein 3, participates in abscisic acid-mediated heat stress resistance in maize" to be published in Journal of Experimental Botany.

Supplementary tables including 4 table:

table s1. 310 DNPs identified in the Mass Spectrometry analysis

table s2.  List of possible interacting proteins of ZmRPP13-LK3 Identified in the IP-MS

table s3.  The primers used in this study

table s4.  Analysis of AC catalytic centers in twenty-one ZmRPP13-LK3 orthologues

Supporting infromation including 10 figures

figure s1 Pie charts showing the distribution of DNPs based on their predicted cellular components
(a), molecular functions (b), biological processes (c) and the signaling pathways (d).

figure s2 Analysis of the protein-protein interaction network among 310 DNPs by using STRING.

figure s3 Sequence alignment of ZmRPP13-LK3 with other known ACs in plant.

figure s4 HPLC method verifying cAMP production catalyzed by purified full-length ZmPSiP or
ZmRPP13-LK3 when using ADP as substrate.

figure s5 HPLC method verifying cAMP production catalyzed by purified full-length ZmPSiP or
ZmRPP13-LK3 when using AMP as substrate.

figure s6 Effect of RNAi against ZmRPP13-LK3 or ZmPSiP in maize protoplasts.

figure s7 Representative total ion chromatogram for cAMP quantification in maize ABA mutant
vp5 and its wild type Vp5 under drought and heat.

figure s8 The relationships between ZmRPP13-LK3 orthologues of several higher plants.

figure s9 The effect of DDA on the activity of ZmPSiP and ZmRPP13-LK3.

figure s10 The relative expressions of ZmRPP13-LK3 and ZmPSiP in maize stems when inoculated
with pathogen.