Published August 18, 2020 | Version v1
Figure Open

Predicted secondary structures (2-D representation of this self-folding) of the terminal untranslated regions (UTR) of FvMV1 and FaMV1-162

Creators

  • 1. Instituto Multidisciplinario de Biología Vegetal (IMBIV), Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET), Avenida Vélez Sarsfield 1611, Córdoba X5016GCA, Córdoba, Argentina and Facultad de Ciencias Exactas Físicas y Naturales (FCEFyN), Universidad Nacional de Córdoba (UNC), Córdoba 5000, Córdoba, Argentina
  • 2. Centro de Investigaciones en Bioquímica Clínica e Inmunología (CIBICI), Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET), Córdoba, Argentina and Departamento de Bioquímica Clínica, Facultad de Ciencias Químicas (FCQ), Universidad Nacional de Córdoba (UNC), Córdoba 5000, Córdoba, Argentina
  • 3. Instituto de Hortofruticultura Subtropical y Mediterránea "La Mayora", Universidad de Málaga, Consejo Superior de Investigaciones Científicas (IHSM – UMA – CSIC), Estación Experimental "La Mayora", Algarrobo-Costa 29750, Málaga, Spain
  • 4. Instituto de Patología Vegetal – Centro de Investigaciones Agropecuarias – Instituto Nacional de Tecnología Agropecuaria (IPAVE – CIAP – INTA), Camino 60 Cuadras Km 5.5, Córdoba X5020ICA, Córdoba, Argentina
  • 5. Estación Experimental Pergamino, (EEA) INTA Pergamino, and Universidad Nacional Noroeste (UNNOBA), Pergamino B2700, Buenos Aires, Argentina

Description

Schemes (a) and (c) represent the 5`-UTR and 3`-UTR of FvMV1 with dG = -82.52 and dG = -29.55, respectively. Schemes (b) and (d) represent the 5`-UTR and 3`-UTR of FaMV1-162 with dG = -73.96 and dG = -13.93, respectively. The +ssRNA molecules were folded, and the free energy was calculated with the RNA Folding Form V 2.3 Energies (MFOLD) program. For these calculations, the following conditions were sectioned: 25ºC, 1M NaCl and 0M divalent ions. The rendering of the structures has been defined with natural angles and annotated using colored base characters, based on p-num information. Colors are ranged from red to black representing the probability as well-determined (1) to poorly determined (0), respectively.

Files

Supplementary Figure 1.tif

Files (2.1 MB)

Name Size Download all
md5:64b1b0212a36e86c5344af5e978a3422
2.1 MB Preview Download